A DNA methylation map of human cancer at single base-pair resolution

Mostra el registre complet Registre parcial de l'ítem

  • dc.contributor.author Vidal Ocabo, Enriqueca
  • dc.contributor.author Sayols, Sergica
  • dc.contributor.author Moran, Sebastianca
  • dc.contributor.author Guillaumet-Adkins, Amyca
  • dc.contributor.author Schroeder, Michael Philipp, 1986-ca
  • dc.contributor.author Royo, Rominaca
  • dc.contributor.author Orozco, Modestoca
  • dc.contributor.author Gut, Martaca
  • dc.contributor.author Gut, Ivo Glynneca
  • dc.contributor.author López Bigas, Núriaca
  • dc.contributor.author Heyn, Holgerca
  • dc.contributor.author Esteller, Manelca
  • dc.date.accessioned 2018-01-19T11:19:22Z
  • dc.date.available 2018-01-19T11:19:22Z
  • dc.date.issued 2017
  • dc.description.abstract Although single base-pair resolution DNA methylation landscapes for embryonic and different somatic cell types provided important insights into epigenetic dynamics and cell-type specificity, such comprehensive profiling is incomplete across human cancer types. This prompted us to perform genome-wide DNA methylation profiling of 22 samples derived from normal tissues and associated neoplasms, including primary tumors and cancer cell lines. Unlike their invariant normal counterparts, cancer samples exhibited highly variable CpG methylation levels in a large proportion of the genome, involving progressive changes during tumor evolution. The whole-genome sequencing results from selected samples were replicated in a large cohort of 1112 primary tumors of various cancer types using genome-scale DNA methylation analysis. Specifically, we determined DNA hypermethylation of promoters and enhancers regulating tumor-suppressor genes, with potential cancer-driving effects. DNA hypermethylation events showed evidence of positive selection, mutual exclusivity and tissue specificity, suggesting their active participation in neoplastic transformation. Our data highlight the extensive changes in DNA methylation that occur in cancer onset, progression and dissemination.
  • dc.description.sponsorship This work was supported by Institute of Health Carlos III ISCIII Project no. PI11/00321, Spanish Cancer Research Network (RTICC) no. RD12/0036/0039; European Development Regional Fund, ‘A way to achieve Europe’ ERDF (SAF2014-55000-R), Sandra Ibarra Foundation; Olga Torres Foundation; Cellex Foundation; AGAUR 2014SGR633 grant; Health and Science Departments of the Catalan government (Generalitat de Catalunya) and European Community's Seventh Framework Program (FP7/2007-2013), grant HEALTH-F5-2011-282510 – BLUEPRINT. HH is a Miguel Servet (CP14/00229) ISCII researcher.
  • dc.format.mimetype application/pdfca
  • dc.identifier.citation Vidal E, Sayols S, Moran S, Guillaumet-Adkins A, Schroeder MP, Royo R et al. A DNA methylation map of human cancer at single base-pair resolution. Oncogene. 2017 Oct 5;36(40):5648-57. DOI: 10.1038/onc.2017.176
  • dc.identifier.doi http://dx.doi.org/10.1038/onc.2017.176
  • dc.identifier.issn 0950-9232
  • dc.identifier.uri http://hdl.handle.net/10230/33697
  • dc.language.iso eng
  • dc.publisher Nature Publishing Groupca
  • dc.relation.ispartof Oncogene. 2017 Oct 5;36(40):5648-57
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/282510
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2014-55000-R
  • dc.rights © Nature Publishing Group. http://dx.doi.org/10.1038/onc.2017.176 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword DNA Methylation
  • dc.subject.keyword Base pairing
  • dc.subject.keyword Neoplasm
  • dc.title A DNA methylation map of human cancer at single base-pair resolutionca
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

Col·leccions

Articles (Departament de Medicina i Ciències de la Vida)
Articles (Center for Genomic Regulation (CRG))
Articles (Hospital del Mar Research Institute)
Documents OpenAIRE (Open Access Infrastructure for Research in Europe)