Genomics reveals introgression and purging of deleterious mutations in the Arabian leopard (Panthera pardus nimr)

Mochales Riaño, Gabriel; Fontseré Alemany, Clàudia, 1992-; Manuel Montero, Marc de, 1991-; Talavera, Adrián; Burriel Carranza, Bernat; Tejero Cicuéndez, Héctor, 1993-; AlGethami, Raed Hamoud M.; Shobrak, Mohammed; Marquès i Bonet, Tomàs, 1975-; Carranza Gil-Dolz del Castellar, Salvador

doi:http://dx.doi.org/10.1016/j.isci.2023.107481

Genomics reveals introgression and purging of deleterious mutations in the Arabian leopard (Panthera pardus nimr)

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Mochales-Riaño G, Fontsere C, de Manuel M, Talavera A, Burriel-Carranza B, Tejero-Cicuéndez H, AlGethami RHM, Shobrak M, Marques-Bonet T, Carranza S. Genomics reveals introgression and purging of deleterious mutations in the Arabian leopard (Panthera pardus nimr). iScience. 2023 Jul 27;26(9):107481. DOI: 10.1016/j.isci.2023.107481

Abstract

In endangered species, low-genetic variation and inbreeding result from recent population declines. Genetic screenings in endangered populations help to assess their vulnerability to extinction and to create informed management actions toward their conservation efforts. The leopard, Panthera pardus, is a highly generalist predator with currently eight different subspecies. Yet, genomic data are still lacking for the Critically Endangered Arabian leopard (P. p. nimr). Here, we sequenced the whole genome of two Arabian leopards and assembled the most complete genomic dataset for leopards to date. Our phylogenomic analyses show that leopards are divided into two deeply divergent clades: the African and the Asian. Conservation genomic analyses indicate a prolonged population decline, which has led to an increase in inbreeding and runs of homozygosity, with consequent purging of deleterious mutations in both Arabian individuals. Our study represents the first attempt to genetically inform captive breeding programmes for this Critically Endangered subspecies.