Id2 GABAergic interneurons comprise a neglected fourth major group of cortical inhibitory cells

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• dc.contributor.author Machold, Robert
• dc.contributor.author Dellal, Shlomo
• dc.contributor.author Valero, Manuel
• dc.contributor.author Zurita, Hector
• dc.contributor.author Kruglikov, Ilya
• dc.contributor.author Meng, John Hongyu
• dc.contributor.author Hanson, Jessica L.
• dc.contributor.author Hashikawa, Yoshiko
• dc.contributor.author Schuman, Benjamin
• dc.contributor.author Buzsáki, György
• dc.contributor.author Rudy, Bernardo
• dc.date.accessioned 2024-05-08T06:58:52Z
• dc.date.available 2024-05-08T06:58:52Z
• dc.date.issued 2023
• dc.description.abstract Cortical GABAergic interneurons (INs) represent a diverse population of mainly locally projecting cells that provide specialized forms of inhibition to pyramidal neurons and other INs. Most recent work on INs has focused on subtypes distinguished by expression of Parvalbumin (PV), Somatostatin (SST), or Vasoactive Intestinal Peptide (VIP). However, a fourth group that includes neurogliaform cells (NGFCs) has been less well characterized due to a lack of genetic tools. Here, we show that these INs can be accessed experimentally using intersectional genetics with the gene Id2. We find that outside of layer 1 (L1), the majority of Id2 INs are NGFCs that express high levels of neuropeptide Y (NPY) and exhibit a late-spiking firing pattern, with extensive local connectivity. While much sparser, non-NGFC Id2 INs had more variable properties, with most cells corresponding to a diverse group of INs that strongly expresses the neuropeptide CCK. In vivo, using silicon probe recordings, we observed several distinguishing aspects of NGFC activity, including a strong rebound in activity immediately following the cortical down state during NREM sleep. Our study provides insights into IN diversity and NGFC distribution and properties, and outlines an intersectional genetics approach for further study of this underappreciated group of INs.
• dc.format.mimetype application/pdf
• dc.identifier.citation Machold R, Dellal S, Valero M, Zurita H, Kruglikov I, Meng JH, et al. Id2 GABAergic interneurons comprise a neglected fourth major group of cortical inhibitory cells. Elife. 2023 Sep 4;12:e85893. DOI: 10.7554/eLife.85893
• dc.identifier.doi http://dx.doi.org/10.7554/eLife.85893
• dc.identifier.issn 2050-084X
• dc.identifier.uri http://hdl.handle.net/10230/60069
• dc.language.iso eng
• dc.publisher eLife
• dc.relation.ispartof Elife. 2023 Sep 4;12:e85893
• dc.rights © 2023, Machold et al. This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword GABAergic Interneurons
• dc.subject.keyword Id2
• dc.subject.keyword Cortical inhibition
• dc.subject.keyword In vivo recordings
• dc.subject.keyword Intersectional genetics
• dc.subject.keyword Mouse
• dc.subject.keyword Neurogliaform cells
• dc.subject.keyword Neuroscience
• dc.title Id2 GABAergic interneurons comprise a neglected fourth major group of cortical inhibitory cells
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion