Hydroxytyrosol, tyrosol and their phase II metabolites as inhibitors of endothelial cell inflammation induced by high glucose levels
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- dc.contributor.author Zodio, Sonia
- dc.contributor.author Serreli, Gabriele
- dc.contributor.author Boronat Rigol, Anna, 1990-
- dc.contributor.author Torre Fornell, Rafael de la
- dc.contributor.author Melis, Maria Paola
- dc.contributor.author Deiana, Monica
- dc.date.accessioned 2025-05-09T05:38:57Z
- dc.date.available 2025-05-09T05:38:57Z
- dc.date.issued 2025
- dc.description.abstract The aim of this investigation was to evaluate the protective effect of the main extra-virgin olive oil (EVOO) phenolic compounds, HT and Tyr, and their phase II metabolites, against the inflammatory response in HUVEC monolayers under hyperglycemic conditions. The alteration of endothelial barrier in HUVEC, treated with high glucose (HG, 30 mM) alone or together with EVOO phenols (1 μM), was evaluated through FITC-Dextran cell permeability test and the determination of TJ proteins occludin, zonulin and JAM-A level, in relation to redox-sensitive MAPK modulation, NLRP3 protein level and cytokines release. Obtained data showed that HG-induced increase of permeability through the alteration of TJ proteins, following the activation of upstream pathways involved in the inflammatory process such as p38, ERK1/2 and NLRP3, was reverted by pre-treatment with EVOO phenols and their metabolites, strengthening the hypothesis that these dietary compounds may exert a significant role in the maintenance of endothelial barrier integrity.
- dc.format.mimetype application/pdf
- dc.identifier.citation Zodio S, Serreli G, Boronat A, De la Torre R, Melis MP, Deiana M. Hydroxytyrosol, tyrosol and their phase II metabolites as inhibitors of endothelial cell inflammation induced by high glucose levels. Journal of Functional Foods. 2025;127:106754. DOI: 10.1016/j.jff.2025.106754
- dc.identifier.doi http://dx.doi.org/10.1016/j.jff.2025.106754
- dc.identifier.issn 1756-4646
- dc.identifier.uri http://hdl.handle.net/10230/70340
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof Journal of Functional Foods. 2025;127:106754
- dc.rights © 2025 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
- dc.subject.keyword Extra-virgin olive oil
- dc.subject.keyword Endothelial cells
- dc.subject.keyword HUVEC
- dc.subject.keyword Hyperglicemia
- dc.subject.keyword Metabolites
- dc.subject.keyword Phenolic compounds
- dc.title Hydroxytyrosol, tyrosol and their phase II metabolites as inhibitors of endothelial cell inflammation induced by high glucose levels
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion