Super resolution imaging of chromatin in pluripotency, differentiation, and reprogramming

Ricci, Maria Aurelia, 1985-; Cosma, Maria Pia, 1970-; Lakadamyali, Melike

doi:http://dx.doi.org/10.1016/j.gde.2017.07.010

Super resolution imaging of chromatin in pluripotency, differentiation, and reprogramming

Citation

Ricci MA, Cosma MP, Lakadamyali M. Super resolution imaging of chromatin in pluripotency, differentiation, and reprogramming. Curr Opin Genet Dev. 2017;46:186-93. DOI 10.1016/j.gde.2017.07.010

Abstract

Chromatin fiber organization is essential for gene function in all cell types. Moreover it helps to determine cell fate in embryonic/adult stem cells and in somatic cells undergoing reprogramming to pluripotency. Until now the diffraction limit of light has limited the inspection of the chromatin fiber organization to a level sufficient to understand how it impacts gene function. The development of advanced microscopy methods, such as single molecule localization microscopy, has largely opened a new field of research providing us with the tools to visualize and quantitatively analyze chromatin fiber organization and thus gene activity at nanoscale resolution in single cells.