Super resolution imaging of chromatin in pluripotency, differentiation, and reprogramming
Super resolution imaging of chromatin in pluripotency, differentiation, and reprogramming
Citació
- Ricci MA, Cosma MP, Lakadamyali M. Super resolution imaging of chromatin in pluripotency, differentiation, and reprogramming. Curr Opin Genet Dev. 2017;46:186-93. DOI 10.1016/j.gde.2017.07.010
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Descripció
Resum
Chromatin fiber organization is essential for gene function in all cell types. Moreover it helps to determine cell fate in embryonic/adult stem cells and in somatic cells undergoing reprogramming to pluripotency. Until now the diffraction limit of light has limited the inspection of the chromatin fiber organization to a level sufficient to understand how it impacts gene function. The development of advanced microscopy methods, such as single molecule localization microscopy, has largely opened a new field of research providing us with the tools to visualize and quantitatively analyze chromatin fiber organization and thus gene activity at nanoscale resolution in single cells.