Generation of cardiomyocytes from new human embrionic stem cell lines derived from poor-quanlity blastocysts

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• dc.contributor.author Raya Chamorro, Ángelca
• dc.contributor.author Rodríguez Pizà, Ignasica
• dc.contributor.author Aran, Begoñaca
• dc.contributor.author Consiglio, Antonellaca
• dc.contributor.author Barri, Pere N.ca
• dc.contributor.author Veiga, Annaca
• dc.contributor.author Izpisúa Belmonte, J. C.ca
• dc.date.accessioned 2012-05-07T11:48:32Z
• dc.date.available 2012-05-07T11:48:32Z
• dc.date.issued 2008ca
• dc.description.abstract Human embryonic stem (hES) cells represent a potential source for cell replacement therapy of many degenerative diseases. Most frequently, hES cell lines are derived from surplus embryos from assisted reproduction cycles, independent of their quality or morphology. Here, we show that hES cell lines can be obtained from poor-quality blastocysts with the same efficiency as that obtained from good- or intermediate-quality blastocysts. Furthermore, we show that the self-renewal, pluripotency, and differentiation ability of hES cell lines derived from either source are comparable. Finally, we present a simple and reproducible embryoid body-based protocol for the differentiation of hES cells into functional cardiomyocytes. The five new hES cell lines derived here should widen the spectrum of available resources for investigating the biology of hES cells and advancing toward efficient strategies of regenerative medicine.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Raya A, Rodríguez-Pizà I, Arán B, Consiglio A, Barri PN, Veiga A, Izpisúa Belmonte JC. Generation of cardiomyocytes from new human embryonic stem cell lines derived from poor-quality blastocysts. Cold Spring Harb Symp Quant Biol. 2008;73:127-35. DOI: 10.1101/sqb.2008.73.038ca
• dc.identifier.doi http://dx.doi.org/10.1101/sqb.2008.73.038
• dc.identifier.issn 0091-7451ca
• dc.identifier.uri http://hdl.handle.net/10230/16403
• dc.language.iso engca
• dc.publisher Cold Spring Harbor Laboratory Press-CSHL Pressca
• dc.relation.ispartof Cold Spring Harbor Symposia on Quantitative Biology. 2008;73:127-35
• dc.rights © 2008, Cold Spring Harbor symposia on quantitative biology by Cold Spring Harbor Laboratory Press. Published version available at http://symposium.cshlp.org/content/73/127.longca
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.other Cél·lules mare embrionàries
• dc.subject.other Medicina regenerativa
• dc.title Generation of cardiomyocytes from new human embrionic stem cell lines derived from poor-quanlity blastocystsca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/submittedVersion