High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development

Bevilacqua, Carlo; Gomez, Juan Manuel; Fiuza, Ulla-Maj; Chan, Chii Jou; Wang, Ling; Hambura, Sebastian; Eguren, Manuel; Ellenberg, Jan; Diz-Muñoz, Alba; Leptin, Maria; Prevedel, Robert

doi:http://dx.doi.org/10.1038/s41592-023-01822-1

High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development

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Bevilacqua C, Gomez JM, Fiuza U, Chan CJ, Wang L, Hambura S, et al. High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development. Nat Methods. 2023 May;20:755-60. DOI: 10.1038/s41592-023-01822-1

Abstract

Brillouin microscopy can assess mechanical properties of biological samples in a three-dimensional (3D), all-optical and hence non-contact fashion, but its weak signals often lead to long imaging times and require an illumination dosage harmful for living organisms. Here, we present a high-resolution line-scanning Brillouin microscope for multiplexed and hence fast 3D imaging of dynamic biological processes with low phototoxicity. The improved background suppression and resolution, in combination with fluorescence light-sheet imaging, enables the visualization of the mechanical properties of cells and tissues over space and time in living organism models such as fruit flies, ascidians and mouse embryos.