Forward-in-time simulation of chromosomal rearrangements: The invisible backbone that sustains long-term adaptation

Banse, Paul; Luiselli, Juliette; Parsons, David P.; Grohens, Théotime; Foley, Marco; Trujillo, Leonardo; Rouzaud-Cornabas, Jonathan; Knibbe, Carole; Beslon, Guillaume

doi:http://dx.doi.org/10.1111/mec.17234

Forward-in-time simulation of chromosomal rearrangements: The invisible backbone that sustains long-term adaptation

License

Citation

Banse P, Luiselli J, Parsons DP, Grohens T, Foley M, Trujillo L, et al. Forward-in-time simulation of chromosomal rearrangements: The invisible backbone that sustains long-term adaptation. Mol Ecol. 2024 Dec;33(24):e17234. DOI: 10.1111/mec.17234

Abstract

While chromosomal rearrangements are ubiquitous in all domains of life, very little is known about their evolutionary significance, mostly because, apart from a few specifically studied and well-documented mechanisms (interaction with recombination, gene duplication, etc.), very few models take them into account. As a consequence, we lack a general theory to account for their direct and indirect contributions to evolution. Here, we propose Aevol, a forward-in-time simulation platform specifically dedicated to unravelling the evolutionary significance of chromosomal rearrangements (CR) compared to local mutations (LM). Using the platform, we evolve populations of organisms in four conditions characterized by an increasing diversity of mutational operators-from substitutions alone to a mix of substitutions, InDels and CR-but with a constant global mutational rate. Despite being almost invisible in the phylogeny owing to the scarcity of their fixation in the lineages, we show that CR make a decisive contribution to the evolutionary dynamics by comparing the outcome in these four conditions. As expected, chromosomal rearrangements allow fast expansion of the gene repertoire through gene duplication, but they also reduce the effect of diminishing-returns epistasis, hence sustaining adaptation on the long-run. At last, we show that chromosomal rearrangements tightly regulate the size of the genome through indirect selection for reproductive robustness. Overall, these results confirm the need to improve our theoretical understanding of the contribution of chromosomal rearrangements to evolution and show that dedicated platforms like Aevol can efficiently contribute to this agenda.