mir-11 limits the proapoptotic function of its host gene, dE2f1

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• dc.contributor.author Truscott, Maryca
• dc.contributor.author Islam, Abul, 1978-ca
• dc.contributor.author López Bigas, Núriaca
• dc.contributor.author Frolov, Maxim V.ca
• dc.date.accessioned 2015-12-18T14:24:09Z
• dc.date.available 2015-12-18T14:24:09Z
• dc.date.issued 2011
• dc.description.abstract The E2F family of transcription factors regulates the expression of both genes associated with cell proliferation and genes that regulate cell death. The net outcome is dependent on cellular context and tissue environment. The mir-11 gene is located in the last intron of the Drosophila E2F1 homolog gene dE2f1, and its expression parallels that of dE2f1. Here, we investigated the role of miR-11 and found that miR-11 specifically modulated the proapoptotic function of its host gene, dE2f1. A mir-11 mutant was highly sensitive to dE2F1-dependent, DNA damage-induced apoptosis. Consistently, coexpression of miR-11 in transgenic animals suppressed dE2F1-induced apoptosis in multiple tissues, while exerting no effect on dE2F1-driven cell proliferation. Importantly, miR-11 repressed the expression of the proapoptotic genes reaper (rpr) and head involution defective (hid), which are directly regulated by dE2F1 upon DNA damage. In addition to rpr and hid, we identified a novel set of cell death genes that was also directly regulated by dE2F1 and miR-11. Thus, our data support a model in which the coexpression of miR-11 limits the proapoptotic function of its host gene, dE2f1, upon DNA damage by directly modulating a dE2F1-dependent apoptotic transcriptional program.ca
• dc.format.mimetype application/pdfca
• dc.identifier.citation Truscott M, Islam AB, López-Bigas N, Frolov MV. mir-11 limits the proapoptotic function of its host gene, dE2f1. Genes & development. 2011;25(17):1820-34. DOI: 10.1101/gad.16947411ca
• dc.identifier.doi http://dx.doi.org/10.1101/gad.16947411
• dc.identifier.issn 0890-9369
• dc.identifier.uri http://hdl.handle.net/10230/25474
• dc.language.iso engca
• dc.publisher Cold Spring Harbor Laboratory Press (CSHL Press)ca
• dc.relation.ispartof Genes & development. 2011;25(17):1820-34
• dc.rights © 2011. Genes and Development by Cold Spring Harbor Laboratory Press. Published version available at http://genesdev.cshlp.org/ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.subject.keyword Cell death
• dc.subject.keyword Apoptosis
• dc.subject.keyword E2F
• dc.subject.keyword MicroRNA
• dc.subject.keyword MiR-11
• dc.subject.keyword DNA damage
• dc.subject.other Drosòfila -- Genèticaca
• dc.subject.other Factors de transcripcióca
• dc.subject.other Cèl·lules HeLaca
• dc.title mir-11 limits the proapoptotic function of its host gene, dE2f1ca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca