Elucidation of the epitranscriptomic RNA modification landscape of Chikungunya Virus

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  • dc.contributor.author Baquero Pérez, Belinda
  • dc.contributor.author Bortoletto, Enrico
  • dc.contributor.author Rosani, Umberto
  • dc.contributor.author Delgado-Tejedor, Anna
  • dc.contributor.author Medina, Rebeca
  • dc.contributor.author Novoa, Eva Maria
  • dc.contributor.author Venier, Paola
  • dc.contributor.author Díez Antón, Juana, 1962-
  • dc.date.accessioned 2024-09-18T13:45:12Z
  • dc.date.available 2024-09-18T13:45:12Z
  • dc.date.issued 2024
  • dc.description.abstract The genomes of positive-sense (+) single-stranded RNA (ssRNA) viruses are believed to be subjected to a wide range of RNA modifications. In this study, we focused on the chikungunya virus (CHIKV) as a model (+) ssRNA virus to study the landscape of viral RNA modification in infected human cells. Among the 32 distinct RNA modifications analysed by mass spectrometry, inosine was found enriched in the genomic CHIKV RNA. However, orthogonal validation by Illumina RNA-seq analyses did not identify any inosine modification along the CHIKV RNA genome. Moreover, CHIKV infection did not alter the expression of ADAR1 isoforms, the enzymes that catalyse the adenosine to inosine conversion. Together, this study highlights the importance of a multidisciplinary approach to assess the presence of RNA modifications in viral RNA genomes.
  • dc.description.sponsorship This work was supported by funds from the Spanish Ministry of Science, Innovation and Universities (PID2022-136939OB-I00 funded by MICIU/AEI/10.13039/501100011033 and ERDF/EU and PID2019106959RB-I00/AEI/10.13039/50110001103), by the 2021 SGR 00176 grant from the Departament de Recerca i Universitats de la Generalitat de Catalunya and an institutional “María de Maeztu” Programme for Units of Excellence in R&D (CEX2018-000792-M). B.B.P. was the recipient of a Beatriu Pinós postdoctoral fellowship funded by the Secretary of Universities and Research (Generalitat de Catalunya) and by the Horizon 2020 programme of research and innovation of the European Union under the Marie Sklodowska-Curie grant agreement No. 801370. The mass spectrometric analyses were performed in the CRG/UPF Proteomics Unit, which is part of the Proteored, PRB3 and is supported by grant PT17/0019, of the PE I+D+i 2013–2016, funded by ISCIII and ERDF. The PhD scholarship to E.B. was granted by the Department of Biology, Univ. of Padova, on funds from the MIUR Project Departments of Excellence.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Baquero-Pérez B, Bortoletto E, Rosani U, Delgado-Tejedor A, Medina R, Novoa EM, et al. Elucidation of the epitranscriptomic RNA modification landscape of Chikungunya Virus. Viruses. 2024 Jun 12;16(6):945. DOI: 10.3390/v16060945
  • dc.identifier.doi http://dx.doi.org/10.3390/v16060945
  • dc.identifier.issn 1999-4915
  • dc.identifier.uri http://hdl.handle.net/10230/61147
  • dc.language.iso eng
  • dc.publisher MDPI
  • dc.relation.ispartof Viruses. 2024 Jun 12;16(6):945
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/801370
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2022-136939OB-I00
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2019-106959RB-I00
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/CEX2018-000792-M
  • dc.rights © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword ADAR1
  • dc.subject.keyword RNA modifications
  • dc.subject.keyword Alphaviruses
  • dc.subject.keyword Chikungunya virus
  • dc.subject.keyword Epitranscriptome
  • dc.subject.keyword Inosine
  • dc.title Elucidation of the epitranscriptomic RNA modification landscape of Chikungunya Virus
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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