Timing of exercise differentially impacts adipose tissue gain in male adolescent rats

Kutsenko, Yevheniy; Iñiguez, Luis P.; Barreda, Alberto; Pardo Marín, Luis; Toval, Angel; Garrigós, Daniel; Martínez Morga, Marta; Pujante, Santiago; Ribeiro Do-Couto, Bruno; Tseng, Kueiyuan; Cerón, José J.; Garaulet, Marta; Wiśniewska, Marta Barbara; Irimia Martínez, Manuel; Ferrán, José Luis E.

doi:http://dx.doi.org/10.1016/j.molmet.2025.102100

Timing of exercise differentially impacts adipose tissue gain in male adolescent rats

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Kutsenko Y, Iñiguez LP, Barreda A, Pardo-Marín L, Toval A, Garrigos D, et al. Timing of exercise differentially impacts adipose tissue gain in male adolescent rats. Mol Metab. 2025 Jan 18;93:102100. DOI: 10.1016/j.molmet.2025.102100

Abstract

Objective: Circadian rhythms of metabolic, hormonal, and behavioral fluctuations and their alterations can impact health. An important gap in knowledge in the field is whether the time of the day of exercise and the age of onset of exercise exert distinct effects at the level of whole-body adipose tissue and body composition. The goal of the present study was to determine how exercise at different times of the day during adolescence impacts the adipose tissue transcriptome and content in a rodent model. Methods: Rats were subjected to one of four conditions during their adolescence: early active phase control or exercise (EAC or EAE; ZT13), and late active phase control or exercise (LAC or LAE; ZT23). The effects of exercise timing were assessed at the level of subcutaneous and visceral adipose tissue transcriptome, body composition, hypothalamic expression of orexigenic and anorexigenic genes, blood serum markers and 24-hour core body temperature patterns. Results: We found that late active phase exercise (ZT23) greatly upregulated pathways of lipid synthesis, glycolysis and NADH shuttles in LAE rats, compared to LAC or EAE. Conversely, LAE rats showed notably lower content of adipose tissue. In addition, LAE rats showed signs of impaired FGF21-adiponectin axis compared to other groups. Conclusions: Finally, LAE rats showed higher post-exercise core body temperature compared to other groups. Our results thus indicate that our exercise protocol induced an unusual effect characterized by enhanced lipid synthesis but reduced adipose tissue content in late active phase but not early active phase exercise during adolescence.