Selective constraints on protamine 2 in primates and rodents

Mostra el registre complet Registre parcial de l'ítem

• dc.contributor.author Lüke, Lenaca
• dc.contributor.author Tourmente, Maximilianoca
• dc.contributor.author Dopazo, Hernánca
• dc.contributor.author Serra, Françoisca
• dc.contributor.author Roldan, Eduardo R. S.ca
• dc.date.accessioned 2016-06-03T14:09:09Z
• dc.date.available 2016-06-03T14:09:09Z
• dc.date.issued 2016
• dc.description.abstract BACKGROUND: Protamines are sperm nuclear proteins with a crucial role in chromatin condensation. Their function is strongly linked to sperm head morphology and male fertility. Protamines appear to be affected by a complex pattern of selective constraints. Previous studies showed that sexual selection affects protamine coding sequence and expression in rodents. Here we analyze selective constraints and post-copulatory sexual selection acting on protamine 2 (Prm2) gene sequences of 53 species of primates and rodents. We focused on possible differences in selective constraints between these two clades and on the two functional domains of PRM2 (cleaved- and mature-PRM2). We also assessed if and how changes in Prm2 coding sequence may affect sperm head dimensions. RESULTS: The domain of Prm2 that is cleaved off during binding to DNA (cleaved-Prm2) was found to be under purifying selection in both clades, whereas the domain that remains bound to DNA (mature-Prm2) was found to be positively selected in primates and under relaxed constraint in rodents. Changes in cleaved-Prm2 coding sequence are significantly correlated to sperm head width and elongation in rodents. Contrary to expectations, a significant effect of sexual selection was not found on either domain or clade. CONCLUSIONS: Mature-PRM2 may be free to evolve under less constraint due to the existence of PRM1 as a more conserved and functionally redundant copy. The cleaved-PRM2 domain seems to play an important role in sperm head shaping. However, sexual selection on its sequence may be difficult to detect until it is identified which sperm head phenotype (shape and size) confers advantages for sperm performance in different mammalian clades.ca
• dc.description.sponsorship This work was supported by grant CGL2011-26341 from the Spanish Ministry of Economy and Competitiveness, a JAE-Predoc studentship from the Spanish National Research Council (CSIC) awarded to L.L., and a Juan de la Cierva postdoctoral fellowship awarded to M.T.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Lüke L, Tourmente M, Dopazo H, Serra F, Roldan ER. Selective constraints on protamine 2 in primates and rodents. BMC evolutionary biology. 2016; 16: 21. DOI 10.1186/s12862-016-0588-1ca
• dc.identifier.doi http://dx.doi.org/10.1186/s12862-016-0588-1
• dc.identifier.issn 1471-2148
• dc.identifier.uri http://hdl.handle.net/10230/26820
• dc.language.iso engca
• dc.publisher BioMed Centralca
• dc.relation.ispartof BMC evolutionary biology. 2016; 16: 21
• dc.rights © 2016 Lüke et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/ca
• dc.subject.keyword Protamine
• dc.subject.keyword Evolution
• dc.subject.keyword Sexual selection
• dc.subject.keyword Sperm competition
• dc.subject.keyword Primates
• dc.subject.keyword Rodents
• dc.subject.keyword Spermatozoa
• dc.subject.other Semenca
• dc.title Selective constraints on protamine 2 in primates and rodentsca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca