Self-sampling monkeypox virus testing in high-risk populations, asymptomatic or with unrecognized Mpox, in Spain

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  • dc.contributor.author Agustí Benito, Cristina
  • dc.contributor.author Adell Lorente, Héctor
  • dc.contributor.author Casabona, Jordi
  • dc.date.accessioned 2025-02-25T07:16:24Z
  • dc.date.available 2025-02-25T07:16:24Z
  • dc.date.issued 2023
  • dc.description.abstract The recent monkeypox virus (MPXV) outbreak was of global concern and has mainly affected gay, bisexual and other men who have sex with men (GBMSM). Here we assess prevalence of MPXV in high-risk populations of GBMSM, trans women (TW) and non-binary people without symptoms or with unrecognized monkeypox (Mpox) symptoms, using a self-sampling strategy. Anal and pharyngeal swabs are tested by MPXV real-time PCR and positive samples are tested for cytopathic effect (CPE) in cell culture. 113 individuals participated in the study, 89 (78.76%) were cis men, 17 (15.04%) were TW. The median age was 35.0 years (IQR: 30.0–43.0), 96 (85.02%) individuals were gay or bisexual and 72 (63.72%) were migrants. Seven participants were MPXV positive (6.19% (95% CI: 1.75%–10.64%)). Five tested positive in pharyngeal swabs, one in anal swab and one in both. Six did not present symptoms recognized as MPXV infection. Three samples were positive for CPE, and showed anti-vaccinia pAb staining by FACS and confocal microscopy. This suggests that unrecognized Mpox cases can shed infectious virus. Restricting testing to individuals reporting Mpox symptoms may not be sufficient to contain outbreaks.en
  • dc.description.sponsorship The authors acknowledge the collaboration of Gema Ballega, Pili Bonamusa, Pamela Nef, Ana Isabel Parra Manzano, Enola Crespillo, Aroa Muñoz, Núria Vaquero, Elisa Molina Molina and F. Hoffmann-La Roche Ltd. In addition, the authors thank Harvey Evans for the English revision. This work was supported by the Ministry of Health of Government of Catalonia (Spain) [no grant number] and Hoffmann-La Roche Laboratories. NI-U is supported by the Spanish Ministry of Science and Innovation (grant PID2020-117145RB-I00), EU HORIZON-HLTH-2021-CORONA-01 (grant 101046118) and by institutional funding from Grifols, Pharma Mar, HIPRA, Amassence and Palobiofarma. Finally, the authors thank the CERCA Programme/Generalitat de Catalunya for their support of the Germans Trias i Pujol Research Institute (IGTP).en
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Agustí C, Martínez-Riveros H, Hernández-Rodríguez À, Casañ C, Díaz Y, Alonso L, et al. Self-sampling monkeypox virus testing in high-risk populations, asymptomatic or with unrecognized Mpox, in Spain. Nat Commun. 2023 Oct 2;14(1):5998. DOI: 10.1038/s41467-023-40490-9
  • dc.identifier.doi http://dx.doi.org/10.1038/s41467-023-40490-9
  • dc.identifier.issn 2041-1723
  • dc.identifier.uri http://hdl.handle.net/10230/69713
  • dc.language.iso eng
  • dc.publisher Springer
  • dc.relation.ispartof Nature Communications. 2023 Oct 2;14(1):5998
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/101046118
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-117145RB-I00
  • dc.rights This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.other Verola del micoca
  • dc.subject.other Verola del mico en humansca
  • dc.subject.other Espanya -- Poblacióca
  • dc.title Self-sampling monkeypox virus testing in high-risk populations, asymptomatic or with unrecognized Mpox, in Spainen
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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