Tuneable endogenous mammalian target complementation via multiplexed plasmid-based recombineering

Beltran-Sastre, Violeta; Benisty, Hannah, 1986-; Burnier, Julia; Berger, Imre; Serrano Pubull, Luis, 1982-; Kiel, Christina

doi:http://dx.doi.org/10.1038/srep17432

Tuneable endogenous mammalian target complementation via multiplexed plasmid-based recombineering

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Beltran-Sastre V, Benisty H, Burnier J, Berger I, Serrano L, Kiel C. Tuneable endogenous mammalian target complementation via multiplexed plasmid-based recombineering. Sci Rep. 2015 Nov 27;5(1):17432. DOI: 10.1038/srep17432

Abstract

Understanding the quantitative functional consequences of human disease mutations requires silencing of endogenous genes and expression of mutants at close to physiological levels. Changing protein levels above or below these levels is also important for system perturbation and modelling. Fast design optimization demands flexible interchangeable cassettes for endogenous gene silencing and tuneable expression. Here, we introduce ‘TEMTAC’, a multigene recombineering and delivery system for simultaneous siRNA-based knockdown and regulated mutant (or other variant) expression with different dynamic ranges. We show its applicability by confirming known phenotypic effects for selected mutations for BRAF, HRAS and SHP2.