Peptides against low density lipoprotein (LDL) aggregation inhibit intracellular cholesteryl ester loading and proliferation of pancreatic tumor cells

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• dc.contributor.author Benitez-Amaro, Aleyda
• dc.contributor.author Martínez Bosch, Neus
• dc.contributor.author Manero Rupérez, Noemí
• dc.contributor.author Claudi, Lene
• dc.contributor.author La Chica Lhoëst, Maria Teresa
• dc.contributor.author Soler, Marta
• dc.contributor.author Ros Blanco, Lia
• dc.contributor.author Navarro Medrano, Pilar
• dc.contributor.author Llorente-Cortés, Vicenta
• dc.date.accessioned 2022-08-03T15:38:48Z
• dc.date.available 2022-08-03T15:38:48Z
• dc.date.issued 2022
• dc.description.abstract Dyslipidemia, metabolic disorders and/or obesity are postulated as risk factors for pancreatic ductal adenocarcinoma (PDAC). The majority of patients with these metabolic alterations have low density lipoproteins (LDLs) with increased susceptibility to become aggregated in the extracellular matrix (ECM). LDL aggregation can be efficiently inhibited by low-density lipoprotein receptor-related protein 1 (LRP1)-based peptides. The objectives of this work were: (i) to determine if aggregated LDLs affect the intracellular cholesteryl ester (CE)/free cholesterol (FC) ratio and/or the tumor pancreatic cell proliferation, using sphingomyelinase-modified LDL particles (Aggregated LDL, AgLDL); and (ii) to test whether LRP1-based peptides, highly efficient against LDL aggregation, can interfere in these processes. For this, we exposed human pancreatic cancer cell lines (PANC-1, RWP-1 and Capan-1) to native (nLDL) or AgLDLs in the absence or presence of LRP1-based peptides (DP3) or irrelevant peptides (IP321). Results of thin-layer chromatography (TLC) following lipid extraction indicate that AgLDLs induce a higher intracellular CE/FC ratio than nLDL, and that DP3 but not IP321 counteracts this effect. AgLDLs also increase PANC-1 cell proliferation, which is inhibited by the DP3 peptide. Our results indicate that AgLDL-induced intracellular CE accumulation plays a crucial role in the proliferation of pancreatic tumor cell lines. Peptides with anti-LDL aggregation properties may thus exhibit anti-tumor effects.
• dc.format.mimetype application/pdf
• dc.identifier.citation Benitez-Amaro A, Martínez-Bosch N, Manero-Rupérez N, Claudi L, La Chica Lhoëst MT, Soler M, Ros-Blanco L, Navarro P, Llorente-Cortés V. Peptides against low density lipoprotein (LDL) aggregation inhibit intracellular cholesteryl ester loading and proliferation of pancreatic tumor cells. Cancers (Basel). 2022 Feb 11;14(4):890. DOI: 10.3390/cancers14040890
• dc.identifier.doi http://dx.doi.org/10.3390/cancers14040890
• dc.identifier.issn 2072-6694
• dc.identifier.uri http://hdl.handle.net/10230/53917
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Cancers (Basel). 2022 Feb 11;14(4):890
• dc.rights © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword LDL atherogenicity
• dc.subject.keyword Anti-LDL aggregation peptides
• dc.subject.keyword Cholesteryl esters
• dc.subject.keyword Pancreatic ductal adenocarcinoma
• dc.title Peptides against low density lipoprotein (LDL) aggregation inhibit intracellular cholesteryl ester loading and proliferation of pancreatic tumor cells
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion