Human genotoxic study carried out two years after oil exposure during the clean-up activities using two different biomarkers
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- dc.contributor.author Biern, Gloria
- dc.contributor.author Giraldo, Jesús
- dc.contributor.author Zock, Jan-Paul
- dc.contributor.author Monyarch, Gemma
- dc.contributor.author Espinosa Díaz, Ana
- dc.contributor.author Rodríguez Trigo, Gema
- dc.contributor.author Gómez, Federico P.
- dc.contributor.author Pozo Rodríguez, Francisco
- dc.contributor.author Fuster, Carme
- dc.contributor.author Barberà, Joan Albert
- dc.contributor.author Fuster, Carme
- dc.date.accessioned 2023-11-20T08:19:15Z
- dc.date.available 2023-11-20T08:19:15Z
- dc.date.issued 2015
- dc.description.abstract Micronuclei, comet and chromosome alterations assays are the most widely used biomarkers for determining the genotoxic damage in a population exposed to genotoxic chemicals. While chromosome alterations are an excellent biomarker to detect short- and long-term genotoxic effects, the comet assay only measures early biological effects, and furthermore it is unknown whether nuclear abnormalies, such as those measured in the micronucleus test, remain detectable long-term after an acute exposure. In our previous study, an increase in structural chromosome alterations in fishermen involved in the clean-up of the Prestige oil spill, two years after acute exposure, was detected. The aim of this study is to investigate whether, in lymphocytes from peripheral blood, the nuclear abnormalies (micronucleus, nucleoplasmic bridges and nuclear buds) have a similar sensitivity to the chromosome damage analysis for genotoxic detection two years after oil exposure in the same non-smoker individuals and in the same peripheral blood extraction. No significant differences in nuclear abnormalies frequencies between exposed and non-exposed individuals were found (p > 0.05). However, chromosome damage, in the same individuals, was higher in exposed vs. non-exposed individuals, especially for chromosome lesions (p < 0.05). These findings, despite the small sample size, suggest that nuclear abnormalities are probably less-successful biomarkers than are chromosome alterations to evaluate genotoxic effects two or more years after an exposure to oil. Due to the great advantage of micronucleus automatic determination, which allows for a rapid study of hundreds of individuals exposed to genotoxic chemical exposure, further studies are needed to confirm whether this assay is or is not useful in long-term genotoxic studies after the toxic agent is no longer present.
- dc.format.mimetype application/pdf
- dc.identifier.citation Biern G, Giraldo J, Zock JP, Monyarch G, Espinosa A, Rodríguez-Trigo G, et al. Human genotoxic study carried out two years after oil exposure during the clean-up activities using two different biomarkers. Jou Mar Sci Eng. 2015 Nov 3;3(4):1334-48. DOI: 10.3390/jmse3041334
- dc.identifier.doi https://doi.org/10.3390/jmse3041334
- dc.identifier.issn 2077-1312
- dc.identifier.uri http://hdl.handle.net/10230/58296
- dc.language.iso eng
- dc.publisher MDPI
- dc.relation.ispartof Journal of Marine Science and Engineering. 2015 Nov 3;3(4):1334-48
- dc.rights © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Micronucleus test
- dc.subject.keyword Chromosome damage
- dc.subject.keyword Nuclear abnormities
- dc.subject.keyword Chromosome alterations
- dc.subject.keyword Oil exposure
- dc.subject.keyword Genotoxicity
- dc.subject.keyword Prestige catastrophe
- dc.title Human genotoxic study carried out two years after oil exposure during the clean-up activities using two different biomarkers
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion