Cross-protection against African swine fever virus upon intranasal vaccination is associated with an adaptive-innate immune crosstalk

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• dc.contributor.author Bosch-Camós, Laia
• dc.contributor.author Alonso, Uxía
• dc.contributor.author Esteve-Codina, Anna
• dc.contributor.author Chang, Chia-Yu
• dc.contributor.author Martín-Mur, Beatriz
• dc.contributor.author Accensi, Francesc
• dc.contributor.author Muñoz, Marta
• dc.contributor.author Navas, María J.
• dc.contributor.author Dabad, Marc
• dc.contributor.author Vidal Barba, Enric
• dc.contributor.author Pina-Pedrero, Sonia
• dc.contributor.author Pleguezuelos, Patricia
• dc.contributor.author Caratù, Ginevra
• dc.contributor.author Salas, María L.
• dc.contributor.author Liu, Lihong
• dc.contributor.author Bataklieva, Stanimira
• dc.contributor.author Gavrilov, Boris
• dc.contributor.author Rodríguez, Fernando
• dc.contributor.author Argilaguet Marqués, Jordi, 1977-
• dc.date.accessioned 2023-01-24T07:20:26Z
• dc.date.available 2023-01-24T07:20:26Z
• dc.date.issued 2022
• dc.description.abstract African swine fever virus (ASFV) is causing a worldwide pandemic affecting the porcine industry and leading to important global economic consequences. The virus causes a highly lethal hemorrhagic disease in wild boars and domestic pigs. Lack of effective vaccines hampers the control of virus spread, thus increasing the pressure on the scientific community for urgent solutions. However, knowledge on the immune components associated with protection is very limited. Here we characterized the in vitro recall response induced by immune cells from pigs intranasally vaccinated with the BA71ΔCD2 deletion mutant virus. Vaccination conferred dose-dependent cross-protection associated with both ASFV-specific antibodies and IFNγ-secreting cells. Importantly, bulk and single-cell transcriptomics of blood and lymph node cells from vaccinated pigs revealed a positive feedback from adaptive to innate immunity. Indeed, activation of Th1 and cytotoxic T cells was concomitant with a rapid IFNγ-dependent triggering of an inflammatory response characterized by TNF-producing macrophages, as well as CXCL10-expressing lymphocytes and cross-presenting dendritic cells. Altogether, this study provides a detailed phenotypic characterization of the immune cell subsets involved in cross-protection against ASFV, and highlights key functional immune mechanisms to be considered for the development of an effective ASF vaccine.
• dc.format.mimetype application/pdf
• dc.identifier.citation Bosch-Camós L, Alonso U, Esteve-Codina A, Chang CY, Martín-Mur B, Accensi F, Muñoz M, Navas MJ, Dabad M, Vidal E, Pina-Pedrero S, Pleguezuelos P, Caratù G, Salas ML, Liu L, Bataklieva S, Gavrilov B, Rodríguez F, Argilaguet J. Cross-protection against African swine fever virus upon intranasal vaccination is associated with an adaptive-innate immune crosstalk. PLoS Pathog. 2022 Nov 9;18(11):e1010931. DOI: 10.1371/journal.ppat.1010931
• dc.identifier.doi http://dx.doi.org/10.1371/journal.ppat.1010931
• dc.identifier.issn 1553-7366
• dc.identifier.uri http://hdl.handle.net/10230/55415
• dc.language.iso eng
• dc.publisher Public Library of Science (PLoS)
• dc.relation.ispartof PLoS Pathog. 2022 Nov 9;18(11):e1010931
• dc.rights © 2022 Bosch-Camós et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Swine
• dc.subject.keyword Viral vaccines
• dc.subject.keyword Immune response
• dc.subject.keyword Vaccination and immunization
• dc.subject.keyword Enzyme-linked immunoassays
• dc.subject.keyword Memory recall
• dc.subject.keyword Antibodies
• dc.subject.keyword Inflammation
• dc.title Cross-protection against African swine fever virus upon intranasal vaccination is associated with an adaptive-innate immune crosstalk
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion