Aurora-A regulates MCRS1 function during mitosis
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- dc.contributor.author Meunier, Sylvainca
- dc.contributor.author Timón Pérez, Krystal, 1987-ca
- dc.contributor.author Vernos, Isabelle, 1959-ca
- dc.date.accessioned 2017-04-19T07:21:00Z
- dc.date.available 2017-04-19T07:21:00Z
- dc.date.issued 2016
- dc.description.abstract The mitotic spindle is made of microtubules (MTs) nucleated through different pathways involving the centrosomes, the chromosomes or the walls of pre-existing MTs. MCRS1 is a RanGTP target that specifically associates with the chromosome-driven MTs protecting them from MT depolymerases. MCRS1 is also needed for the control of kinetochore fiber (K-fiber) MT minus-ends dynamics in metaphase. Here, we investigated the regulation of MCRS1 activity in M-phase. We show that MCRS1 is phosphorylated by the Aurora-A kinase in mitosis on Ser35/36. Although this phosphorylation has no role on MCRS1 localization to chromosomal MTs and K-fiber minus-ends, we show that it regulates MCRS1 activity in mitosis. We conclude that Aurora-A activity is particularly important in the tuning of K-fiber minus-ends dynamics in mitosis.
- dc.description.sponsorship Work in the Vernos lab is supported by the Spanish Ministry of Economy and Competitiveness grants BFU2012-37163. We acknowledge support of the Spanish Ministry of Economy and Competitiveness, “Centro de Excelencia Severo Ochoa 2013–2017”, SEV-2012-0208.
- dc.format.mimetype application/pdfca
- dc.identifier.citation Meunier S, Timón Pérez K, Vernos I. Aurora-A regulates MCRS1 function during mitosis. Cell Cycle. 2016;15(13):1779-86. DOI: 10.1080/15384101.2016.1187342
- dc.identifier.doi http://dx.doi.org/10.1080/15384101.2016.1187342
- dc.identifier.issn 1538-4101
- dc.identifier.uri http://hdl.handle.net/10230/30833
- dc.language.iso eng
- dc.publisher Taylor & Francis (Routledge)ca
- dc.relation.ispartof Cell Cycle. 2016;15(13):1779-86
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SEV2012-0208
- dc.rights © 2016 The Author(s). Published with license by Taylor & Francis.© Sylvain Meunier, Krystal Timon, and Isabelle Vernos. This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc/3.0/
- dc.subject.keyword Aurora-A kinase
- dc.subject.keyword Chromosomal microtubules
- dc.subject.keyword K-fiber
- dc.subject.keyword MCRS1
- dc.subject.keyword Microtubule minus-end
- dc.subject.keyword Mitosis
- dc.subject.keyword Phosphorylation
- dc.title Aurora-A regulates MCRS1 function during mitosisca
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion