Aurora-A regulates MCRS1 function during mitosis

Meunier, Sylvain; Timón Pérez, Krystal, 1987-; Vernos, Isabelle, 1959-

doi:http://dx.doi.org/10.1080/15384101.2016.1187342

Aurora-A regulates MCRS1 function during mitosis

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Meunier S, Timón Pérez K, Vernos I. Aurora-A regulates MCRS1 function during mitosis. Cell Cycle. 2016;15(13):1779-86. DOI: 10.1080/15384101.2016.1187342

Abstract

The mitotic spindle is made of microtubules (MTs) nucleated through different pathways involving the centrosomes, the chromosomes or the walls of pre-existing MTs. MCRS1 is a RanGTP target that specifically associates with the chromosome-driven MTs protecting them from MT depolymerases. MCRS1 is also needed for the control of kinetochore fiber (K-fiber) MT minus-ends dynamics in metaphase. Here, we investigated the regulation of MCRS1 activity in M-phase. We show that MCRS1 is phosphorylated by the Aurora-A kinase in mitosis on Ser35/36. Although this phosphorylation has no role on MCRS1 localization to chromosomal MTs and K-fiber minus-ends, we show that it regulates MCRS1 activity in mitosis. We conclude that Aurora-A activity is particularly important in the tuning of K-fiber minus-ends dynamics in mitosis.