Promoter nucleosome dynamics regulated by signalling through the CTD code
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- dc.contributor.author Materne, Philippeca
- dc.contributor.author Anandhakumar, Jayamanica
- dc.contributor.author Migeot, Valerieca
- dc.contributor.author Soriano, Ignacioca
- dc.contributor.author Yague Sanz, Carloca
- dc.contributor.author Hidalgo Hernando, Elenaca
- dc.contributor.author Mignion, Caroleca
- dc.contributor.author Quintales, Luisca
- dc.contributor.author Antequera, Franciscoca
- dc.contributor.author Hermand, Damienca
- dc.date.accessioned 2016-02-01T14:41:09Z
- dc.date.available 2016-02-01T14:41:09Z
- dc.date.issued 2015
- dc.description.abstract The phosphorylation of the RNA polymerase II C-terminal domain (CTD) plays a key role in delineating transcribed regions within chromatin by recruiting histone methylases and deacetylases. Using genome-wide nucleosome mapping, we show that CTD S2 phosphorylation controls nucleosome dynamics in the promoter of a subset of 324 genes, including the regulators of cell differentiation ste11 and metabolic adaptation inv1. Mechanistic studies on these genes indicate that during gene activation a local increase of phospho-S2 CTD nearby the promoter impairs the phospho-S5 CTD-dependent recruitment of Set1 and the subsequent recruitment of specific HDACs, which leads to nucleosome depletion and efficient transcription. The early increase of phospho-S2 results from the phosphorylation of the CTD S2 kinase Lsk1 by MAP kinase in response to cellular signalling. The artificial tethering of the Lsk1 kinase at the ste11 promoter is sufficient to activate transcription. Therefore, signalling through the CTD code regulates promoter nucleosomes dynamics.ca
- dc.description.sponsorship This work was supported by grants BFU2011-28804 and Consolider Ingenio CSD2007-00015 from the Spanish Ministerio de Economía y Competitividad to FA, and grants FRFC 2.4510.10, Credit aux chercheurs 1.5.013.09, MIS F.4523.11, Ceruna and Marie Curie Action to DH. DH is a FNRS Research Associate
- dc.format.mimetype application/pdfca
- dc.identifier.citation Materne P, Anandhakumar J, Migeot V, Soriano I, Yague-Sanz C, Hidalgo E et al. Promoter nucleosome dynamics regulated by signalling through the CTD code. Elife. 2015;4:e09008. DOI: 10.7554/eLife.09008ca
- dc.identifier.doi http://dx.doi.org/10.7554/eLife.09008
- dc.identifier.issn 2050-084X
- dc.identifier.uri http://hdl.handle.net/10230/25707
- dc.language.iso engca
- dc.publisher eLifeca
- dc.relation.ispartof Elife. 2015;4:e09008
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/CSD2007-00015
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/BFU2011-28804
- dc.rights © Copyright Materne et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.ca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/ca
- dc.subject.keyword HDAC
- dc.subject.keyword MAP kinase
- dc.subject.keyword RNA polymerase
- dc.subject.keyword S. pombe
- dc.subject.keyword Set1
- dc.subject.keyword Chromatin
- dc.subject.keyword Chromosomes
- dc.subject.keyword Genes
- dc.subject.other RNA-polimerasesca
- dc.subject.other Cromatinaca
- dc.title Promoter nucleosome dynamics regulated by signalling through the CTD codeca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca