Ataxin-2, Twenty-four, and Dicer-2 are components of a noncanonical cytoplasmic polyadenylation complex

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  • dc.contributor.author Nadimpalli, Hima Priyanka, 1988-
  • dc.contributor.author Guitart, Tanit
  • dc.contributor.author Coll, Olga
  • dc.contributor.author Gebauer, Fátima
  • dc.date.accessioned 2022-11-03T07:10:26Z
  • dc.date.available 2022-11-03T07:10:26Z
  • dc.date.issued 2022
  • dc.description.abstract Cytoplasmic polyadenylation is a mechanism to promote mRNA translation in a wide variety of biological contexts. A canonical complex centered around the conserved RNA-binding protein family CPEB has been shown to be responsible for this process. We have previously reported evidence for an alternative noncanonical, CPEB-independent complex in Drosophila, of which the RNA-interference factor Dicer-2 is a component. Here, we investigate Dicer-2 mRNA targets and protein cofactors in cytoplasmic polyadenylation. Using RIP-Seq analysis, we identify hundreds of potential Dicer-2 target transcripts, ∼60% of which were previously found as targets of the cytoplasmic poly(A) polymerase Wispy, suggesting widespread roles of Dicer-2 in cytoplasmic polyadenylation. Large-scale immunoprecipitation revealed Ataxin-2 and Twenty-four among the high-confidence interactors of Dicer-2. Complex analyses indicated that both factors form an RNA-independent complex with Dicer-2 and mediate interactions of Dicer-2 with Wispy. Functional poly(A)-test analyses showed that Twenty-four and Ataxin-2 are required for cytoplasmic polyadenylation of a subset of Dicer-2 targets. Our results reveal components of a novel cytoplasmic polyadenylation complex that operates during Drosophila early embryogenesis.
  • dc.description.sponsorship This work was supported by grants from the Spanish Ministry of Science and Innovation (PGC2018-099697-B-I00 and BFU2015-68741) and the Catalan Agency for Research and Universities (2017SGR534). We acknowledge the support of the Spanish Ministry of Science and Innovation through the Centro de Excelencia Severo Ochoa (CEX2020-001049-S, MCIN/AEI/10.13039/501100011033) and the Generalitat de Catalunya through the CERCA Programme.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Nadimpalli HP, Guitart T, Coll O, Gebauer F. Ataxin-2, Twenty-four, and Dicer-2 are components of a noncanonical cytoplasmic polyadenylation complex. Life Sci Alliance. 2022 Sep 16;5(12):e202201417. DOI: 10.26508/lsa.202201417
  • dc.identifier.doi http://dx.doi.org/10.26508/lsa.202201417
  • dc.identifier.issn 2575-1077
  • dc.identifier.uri http://hdl.handle.net/10230/54664
  • dc.language.iso eng
  • dc.publisher Life Science Alliance
  • dc.relation.ispartof Life Sci Alliance. 2022 Sep 16;5(12):e202201417
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-099697-B-I00
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-68741
  • dc.rights © 2022 Nadimpalli et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri https://creativecommons.org/licenses/by/4.0/
  • dc.title Ataxin-2, Twenty-four, and Dicer-2 are components of a noncanonical cytoplasmic polyadenylation complex
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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