Transcriptomic taxonomy and neurogenic trajectories of adult human, macaque, and pig hippocampal and entorhinal cells
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- dc.contributor.author Franjic, Daniel
- dc.contributor.author Santpere Baró, Gabriel, 1981-
- dc.contributor.author Sestan, Nenad
- dc.date.accessioned 2022-09-20T07:59:00Z
- dc.date.available 2022-09-20T07:59:00Z
- dc.date.issued 2022
- dc.description.abstract The hippocampal-entorhinal system supports cognitive functions, has lifelong neurogenic capabilities in many species, and is selectively vulnerable to Alzheimer's disease. To investigate neurogenic potential and cellular diversity, we profiled single-nucleus transcriptomes in five hippocampal-entorhinal subregions in humans, macaques, and pigs. Integrated cross-species analysis revealed robust transcriptomic and histologic signatures of neurogenesis in the adult mouse, pig, and macaque but not humans. Doublecortin (DCX), a widely accepted marker of newly generated granule cells, was detected in diverse human neurons, but it did not define immature neuron populations. To explore species differences in cellular diversity and implications for disease, we characterized subregion-specific, transcriptomically defined cell types and transitional changes from the three-layered archicortex to the six-layered neocortex. Notably, METTL7B defined subregion-specific excitatory neurons and astrocytes in primates, associated with endoplasmic reticulum and lipid droplet proteins, including Alzheimer's disease-related proteins. This resource reveals cell-type- and species-specific properties shaping hippocampal-entorhinal neurogenesis and function.
- dc.format.mimetype application/pdf
- dc.identifier.citation Franjic D, Skarica M, Ma S, Arellano JI, Tebbenkamp ATN, Choi J, et al. Transcriptomic taxonomy and neurogenic trajectories of adult human, macaque, and pig hippocampal and entorhinal cells. Neuron. 2022 Feb 2; 110(3): 452-69. e14. DOI: 10.1016/j.neuron.2021.10.036
- dc.identifier.doi http://dx.doi.org/10.1016/j.neuron.2021.10.036
- dc.identifier.issn 0896-6273
- dc.identifier.uri http://hdl.handle.net/10230/54119
- dc.language.iso eng
- dc.publisher Elsevier
- dc.rights © 2021, Franjic D, Skarica M, Ma S, Arellano JI, Tebbenkamp ATN, Choi J. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
- dc.subject.keyword Alzheimer’s disease
- dc.subject.keyword METTL7B
- dc.subject.keyword Adult neurogenesis
- dc.subject.keyword Doublecortin
- dc.subject.keyword Entorhinal cortex
- dc.subject.keyword Evolution
- dc.subject.keyword Hippocampus
- dc.subject.keyword Immature neurons
- dc.subject.keyword Neuroblast
- dc.subject.keyword Single-cell RNA-seq
- dc.title Transcriptomic taxonomy and neurogenic trajectories of adult human, macaque, and pig hippocampal and entorhinal cells
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion