Nf-core/reportho: a pipeline for comparative analysis of ortholog predictions

Trujnara, Igor

Nf-core/reportho: a pipeline for comparative analysis of ortholog predictions

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Trujnara, I. Nf-core/reportho: a pipeline for comparative analysis of ortholog predictions. 2024. handle: http://hdl.handle.net/10230/68908

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Abstract

Orthologous genes are crucial for the study of protein function and evolution. Multiple methods have been created to predict orthologs. Quest for Orthologs has benchmarked those methods but has not created a comprehensive prediction comparison. We propose nf-core/reportho, a pipeline that retrieves public ortholog predictions, performs systematic comparisons, calculates agreement statistics, and presents them in a human-readable format. The pipeline shows satisfactory performance and strong scalability. A run on a representative sample of human proteins demonstrates limited agreement between sources and highlights challenges for the field, especially in the aspect of data integration.