Selective cleavage of ncRNA and antiviral activity by RNase2/EDN in THP1-induced macrophages

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• dc.contributor.author Lu, Lu
• dc.contributor.author Li, Jiarui
• dc.contributor.author Wei, Ranlei
• dc.contributor.author Guidi, Irene
• dc.contributor.author Cozzuto, Luca
• dc.contributor.author Ponomarenko, Julia
• dc.contributor.author Prats-Ejarque, Guillem
• dc.contributor.author Boix, Ester
• dc.date.accessioned 2022-05-25T10:39:31Z
• dc.date.available 2022-05-25T10:39:31Z
• dc.date.issued 2022
• dc.description.abstract RNase2 is the member of the RNaseA family most abundant in macrophages. Here, we knocked out RNase2 in THP-1 cells and analysed the response to Respiratory Syncytial Virus (RSV). RSV induced RNase2 expression, which significantly enhanced cell survival. Next, by cP-RNAseq sequencing, which amplifies the cyclic-phosphate endonuclease products, we analysed the ncRNA population. Among the ncRNAs accumulated in WT vs KO cells, we found mostly tRNA-derived fragments (tRFs) and second miRNAs. Differential sequence coverage identified tRFs from only few parental tRNAs, revealing a predominant cleavage at anticodon and D-loops at U/C (B1) and A (B2) sites. Selective tRNA cleavage was confirmed in vitro using the recombinant protein. Likewise, only few miRNAs were significantly more abundant in WT vs RNase2-KO cells. Complementarily, by screening of a tRF & tiRNA array, we identified an enriched population associated to RNase2 expression and RSV exposure. The results confirm the protein antiviral action and provide the first evidence of its cleavage selectivity on ncRNAs.
• dc.description.sponsorship Funding: Open Access Funding provided by Universitat Autònoma de Barcelona. This research was founded by Research work was supported by the Ministerio de Economía y Competitividad (SAF2015-66007P), co-financed by FEDER funds, by Agencia Estatal de Investigación (PID2019-106123GB-I00/AEI/10.13039/501100011033) and by Fundació La Marató de TV3 (2080310). LL and JL were supported by China Scholarship Council (CSC) predoctoral fellowships
• dc.format.mimetype application/pdf
• dc.identifier.citation Lu L, Li J, Wei R, Guidi I, Cozzuto L, Ponomarenko J et al. Selective cleavage of ncRNA and antiviral activity by RNase2/EDN in THP1-induced macrophages. Cell Mol Life Sci. 2022 Mar 26;79(4):209. DOI:10.1007/s00018-022-04229-x
• dc.identifier.doi http://dx.doi.org/10.1007/s00018-022-04229-x
• dc.identifier.issn 1420-682X
• dc.identifier.uri http://hdl.handle.net/10230/53264
• dc.language.iso eng
• dc.publisher Springer
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2015-66007P
• dc.rights © Lu Lu et al. 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.subject.other Genètica
• dc.subject.other Virus
• dc.subject.other Virus sincicial respiratori
• dc.subject.other Ribonucleases
• dc.title Selective cleavage of ncRNA and antiviral activity by RNase2/EDN in THP1-induced macrophages
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion