Dissection of a redox relay: H2O2-dependent activation of the transcription factor pap1 through the peroxidatic tpx1-thioredoxin cycle

Calvo, Isabel A.; Boronat i Llop, Susanna, 1965-; Domènech, Alba; García Santamarina, Sarela, 1978-; Ayté del Olmo, José; Hidalgo Hernando, Elena

Dissection of a redox relay: H2O2-dependent activation of the transcription factor pap1 through the peroxidatic tpx1-thioredoxin cycle

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dc.contributor.author Calvo, Isabel A.ca
dc.contributor.author Boronat i Llop, Susanna, 1965-ca
dc.contributor.author Domènech, Albaca
dc.contributor.author García Santamarina, Sarela, 1978-ca
dc.contributor.author Ayté del Olmo, Joséca
dc.contributor.author Hidalgo Hernando, Elenaca
dc.date.accessioned 2015-06-04T06:56:43Z
dc.date.available 2015-06-04T06:56:43Z
dc.date.issued 2013ca
dc.description.abstract In fission yeast, the transcription factor Pap1 undergoes H2O2-dependent oxidation that promotes its nuclear accumulation and the activation of an antioxidant gene program. However, the mechanisms that regulate the sensitivity and selectivity of Pap1 activation by peroxides are not fully understood. Here, we demonstrate that the peroxiredoxin Tpx1, the sensor of this signaling cascade, activates the otherwise unresponsive Pap1 protein once the main cytosolic reduced thioredoxin, Trx1, becomes transiently depleted. In other words, Pap1 works as an alternative electron donor for oxidized Tpx1. We have trapped the very transient Tpx1-Pap1 intermediate in cells depleted in Trx1, as we show here using mass spectrometry. Recycling of Tpx1 by Trx1 is required for the efficient signaling to Pap1, suggesting that the complete cycle of H2O2 scavenging by Tpx1 and further recycling of oxidized Tpx1 by Trx1 is required for full downstream activation of the redox cascade.en
dc.description.sponsorship This work was supported by the Spanish Ministry of Science and Innovation (BFU2009-06933 and BFU2012-32045), PLAN E and FEDER, by the Spanish program Consolider-Ingenio 2010 grant CSD 2007-0020, and by SGR2009-195 from Generalitat de Catalunya (Spain) to E.H. E.H. and J.A. are recipients of ICREA Academia Awards (Generalitat de Catalunya)en
dc.format.mimetype application/pdfca
dc.identifier.citation Calvo I, Boronat S, Domenech A, Garcia-Santamarina S, Ayté J, Hidalgo E. Dissection of a redox relay: H2O2-dependent activation of the transcription factor pap1 through the peroxidatic tpx1-thioredoxin cycle. Cell Reports. 2013;5(5):1413-24. DOI: 10.1016/j.celrep.2013.11.027ca
dc.identifier.doi http://dx.doi.org/10.1016/j.celrep.2013.11.027
dc.identifier.issn 2211-1247ca
dc.identifier.uri http://hdl.handle.net/10230/23724
dc.language.iso engca
dc.publisher Elsevierca
dc.relation.ispartof Cell Reports. 2013;5(5):1413-24
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2009-06933
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-32045
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/CSD2007-0020
dc.rights © 2013, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 International http://creativecommons.org/licenses/by-nc-nd/3.0/ http://dx.doi.org/10.1016/j.celrep.2013.11.027en
dc.rights.accessRights info:eu-repo/semantics/openAccessca
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.other Schizosaccharomyces pombe -- Metabolismeca
dc.subject.other Peroxidasa -- Metabolismeca
dc.title Dissection of a redox relay: H2O2-dependent activation of the transcription factor pap1 through the peroxidatic tpx1-thioredoxin cycleen
dc.type info:eu-repo/semantics/articleca
dc.type.version info:eu-repo/semantics/acceptedVersionca

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