What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry
Mostra el registre complet Registre parcial de l'ítem
- dc.contributor.author Borràs, Evaca
- dc.contributor.author Sabidó Aguadé, Eduard, 1981-ca
- dc.date.accessioned 2018-06-14T07:43:19Z
- dc.date.issued 2017
- dc.description.abstract Targeted proteomics has gained significant popularity in mass spectrometry-based protein quantification as a method to detect proteins of interest with high sensitivity, quantitative accuracy and reproducibility. However, with the emergence of a wide variety of targeted proteomics methods, some of them with high-throughput capabilities, it is easy to overlook the essence of each method and to determine what makes each of them a targeted proteomics method. In this viewpoint, we revisit the main targeted proteomics methods and classify them in four categories differentiating those methods that perform targeted data acquisition from targeted data analysis, and those methods that are based on peptide ion data (MS1 targeted methods) from those that rely on the peptide fragments (MS2 targeted methods).
- dc.format.mimetype application/pdf
- dc.identifier.citation Borràs E, Sabidó E. What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry. Proteomics. 2017 Sep;17(17-18):1700180. DOI: 10.1002/pmic.201700180
- dc.identifier.doi http://dx.doi.org/10.1002/pmic.201700180
- dc.identifier.issn 1615-9853
- dc.identifier.uri http://hdl.handle.net/10230/34896
- dc.language.iso eng
- dc.publisher Wileyca
- dc.relation.ispartof Proteomics. 2017 Sep;17(17-18):1700180
- dc.rights This is the peer reviewed version of the following article: Borràs E, Sabidó E. What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry. Proteomics. 2017 Sep;17(17-18):1700180, which has been published in final form at http://dx.doi.org/10.1002/pmic.201700180. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.keyword Data independent acquisition
- dc.subject.keyword Parallel reaction monitoring
- dc.subject.keyword Mass spectrometry
- dc.subject.keyword Targeted proteomics
- dc.subject.keyword Selected reaction monitoring
- dc.title What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometryca
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/acceptedVersion