High-throughput RNA sequencing of the T cell receptor alpha and beta chains for simultaneous clonality and biological analyses in Sezary syndrome

Mostra el registre complet Registre parcial de l'ítem

  • dc.contributor.author Blanco Ares, Gonzalo, 1989-
  • dc.contributor.author López-Aventín, Daniel
  • dc.contributor.author Pujol Vallverdú, Ramon Maria
  • dc.contributor.author Gómez-Llonín, Andrea
  • dc.contributor.author Puiggros Metje, Anna Maria
  • dc.contributor.author López-Sánchez, Manuela
  • dc.contributor.author Estrach, Teresa
  • dc.contributor.author García-Muret, Mª Pilar
  • dc.contributor.author López Lerma, Ingrid
  • dc.contributor.author Servitje, Octavio
  • dc.contributor.author Bellosillo Paricio, Beatriz
  • dc.contributor.author Muro, Manuel
  • dc.contributor.author Espinet Solà, Blanca
  • dc.contributor.author Rabionet, Raquel
  • dc.contributor.author Gallardo Hernández, Fernando
  • dc.date.accessioned 2024-10-23T06:09:22Z
  • dc.date.available 2024-10-23T06:09:22Z
  • dc.date.issued 2023
  • dc.description.abstract Background: Previous investigations pointed out a role for antigen stimulation in Sezary syndrome (SS). High-throughput sequencing of the T cell receptor (TR) offers several applications beyond diagnostic purposes, including the study of T cell pathogenesis. Methods: We performed high-throughput RNA sequencing of the TR alpha (TRA) and beta (TRB) genes focusing on the complementarity-determining region 3 (CDR3) in 11 SS and one erythrodermic mycosis fungoides (MF) patients. Five psoriasis patients were employed as controls. Peripheral blood CD4+ cells were isolated and RNA sequenced (HiSeq2500). High-resolution HLA typing was performed in neoplastic patients. Results: Highly expanded predominant TRA and TRB CDR3 were only found in SS patients (median frequency: 94.4% and 93.7%). No remarkable CDR3 expansions were observed in psoriasis patients (median frequency of predominant TRA and TRB CDR3: 0.87% and 0.69%, p < 0.001 compared to SS). CDR3 almost identical to the predominant were identified within each SS patient and were exponentially correlated with frequencies of the predominant CDR3 (R2 = 0.918, p < 0.001). Forty-six different CDR3 were shared between SS patients displaying HLA similarities, including predominant TRA and TRB CDR3 in one patient that were found in other three patients. Additionally, 351 antigen matches were detected (Cytomegalovirus, Epstein-Barr, Influenza virus, and self-antigens), and the predominant CDR3 of two different SS patients matched CDR3 with specificity for Influenza and Epstein-Barr viruses. Conclusions: Besides detecting clonality, these findings shed light on the nature of SS-related antigens, pointing to RNA sequencing as a useful tool for simultaneous clonality and biological analysis in SS.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Blanco G, López-Aventín D, Pujol RM, Gómez-Llonín A, Puiggros A, López-Sánchez M, et al. High-throughput RNA sequencing of the T cell receptor alpha and beta chains for simultaneous clonality and biological analyses in Sezary syndrome. J Clin Lab Anal. 2023 Dec;37(23-24):e24982. DOI: 10.1002/jcla.24982
  • dc.identifier.doi http://dx.doi.org/10.1002/jcla.24982
  • dc.identifier.issn 0887-8013
  • dc.identifier.uri http://hdl.handle.net/10230/68317
  • dc.language.iso eng
  • dc.publisher Wiley
  • dc.relation.ispartof J Clin Lab Anal. 2023 Dec;37(23-24):e24982
  • dc.rights © 2023 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
  • dc.subject.keyword RNA sequencing
  • dc.subject.keyword Sezary syndrome
  • dc.subject.keyword T cell receptor
  • dc.subject.keyword Antigen restriction
  • dc.subject.keyword Complementarity determining region
  • dc.subject.keyword Mycosis fungoides
  • dc.title High-throughput RNA sequencing of the T cell receptor alpha and beta chains for simultaneous clonality and biological analyses in Sezary syndrome
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

Col·leccions

Articles (Hospital del Mar Research Institute)