Relaxation of selective constraints causes independent selenoprotein extinction in insect genomes

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• dc.contributor.author Guigó Serra, Rodericca
• dc.contributor.author Chapple, Charles E.ca
• dc.date.accessioned 2010-06-10T14:41:19Z
• dc.date.available 2010-06-10T14:41:19Z
• dc.date.issued 2008ca
• dc.description.abstract BACKGROUND: Selenoproteins are a diverse family of proteins notable for the presence of the 21st amino acid, selenocysteine. Until very recently, all metazoan genomes investigated encoded selenoproteins, and these proteins had therefore been believed to be essential for animal life. Challenging this assumption, recent comparative analyses of insect genomes have revealed that some insect genomes appear to have lost selenoprotein genes. METHODOLOGY/PRINCIPAL FINDINGS: In this paper we investigate in detail the fate of selenoproteins, and that of selenoprotein factors, in all available arthropod genomes. We use a variety of in silico comparative genomics approaches to look for known selenoprotein genes and factors involved in selenoprotein biosynthesis. We have found that five insect species have completely lost the ability to encode selenoproteins and that selenoprotein loss in these species, although so far confined to the Endopterygota infraclass, cannot be attributed to a single evolutionary event, but rather to multiple, independent events. Loss of selenoproteins and selenoprotein factors is usually coupled to the deletion of the entire no-longer functional genomic region, rather than to sequence degradation and consequent pseudogenisation. Such dynamics of gene extinction are consistent with the high rate of genome rearrangements observed in Drosophila. We have also found that, while many selenoprotein factors are concomitantly lost with the selenoproteins, others are present and conserved in all investigated genomes, irrespective of whether they code for selenoproteins or not, suggesting that they are involved in additional, non-selenoprotein related functions. CONCLUSIONS/SIGNIFICANCE: Selenoproteins have been independently lost in several insect species, possibly as a consequence of the relaxation in insects of the selective constraints acting across metazoans to maintain selenoproteins. The dispensability of selenoproteins in insects may be related to the fundamental differences in antioxidant defense between these animals and other metazoans.
• dc.description.sponsorship The work described here is funded by grants from the Spanish Ministery of Education and Science and from the BioSapiens European Network of Excellence to RG. CEC is reciepient of a pre-doctoral fellowship from the Spanish Ministery of Education and Science.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Chapple CE, Guigó R. Relaxation of selective constraints causes independent selenoprotein extinction in insect genomes. PLoS ONE. 2008; 3(8):e2968. DOI: 10.1371/journal.pone.0002968
• dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0002968
• dc.identifier.issn 1932-6203
• dc.identifier.uri http://hdl.handle.net/10230/6240
• dc.language.iso engca
• dc.publisher Public Library of Science (PLoS)ca
• dc.relation.ispartof PLoS ONE. 2008;3(8):e2968
• dc.rights (c) 2008 Chapple et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/2.5/
• dc.subject.keyword Animals
• dc.subject.keyword Biological extinction
• dc.subject.keyword Genome
• dc.subject.keyword Selenocysteine
• dc.subject.keyword Selenoproteins
• dc.subject.other Selenocisteïna -- Aspectes genètics
• dc.title Relaxation of selective constraints causes independent selenoprotein extinction in insect genomesca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersion