Influence of perinatal and childhood exposure to tobacco and mercury in children's gut microbiota

Mostra el registre complet Registre parcial de l'ítem

• dc.contributor.author Pérez Castro, Sonia
• dc.contributor.author D'Auria, Giussepe
• dc.contributor.author Llambrich, Maria
• dc.contributor.author Fernández-Barrés, Sílvia
• dc.contributor.author Lopez-Espinosa, Maria-José
• dc.contributor.author Llop, Sabrina
• dc.contributor.author Regueiro, Benito
• dc.contributor.author Bustamante Pineda, Mariona
• dc.contributor.author Francino, M. Pilar
• dc.contributor.author Vrijheid, Martine
• dc.contributor.author Maitre, Léa
• dc.date.accessioned 2024-06-27T14:27:21Z
• dc.date.available 2024-06-27T14:27:21Z
• dc.date.issued 2024
• dc.description.abstract Background: Early life determinants of the development of gut microbiome composition in infants have been widely investigated; however, if early life pollutant exposures, such as tobacco or mercury, have a persistent influence on the gut microbial community, its stabilization at later childhood remains largely unknown. Objective: In this exposome-wide study, we aimed at identifying the contribution of exposure to tobacco and mercury from the prenatal period to childhood, to individual differences in the fecal microbiome composition of 7-year-old children, considering co-exposure to a width of established lifestyle and clinical determinants. Methods: Gut microbiome was studied by 16S rRNA amplicon sequencing in 151 children at the genus level. Exposure to tobacco was quantified during pregnancy through questionnaire (active tobacco consumption, second-hand smoking -SHS) and biomonitoring (urinary cotinine) at 4 years (urinary cotinine, SHS) and 7 years (SHS). Exposure to mercury was quantified during pregnancy (cord blood) and at 4 years (hair). Forty nine other potential environmental determinants (12 at pregnancy/birth/infancy, 15 at 4 years and 22 at 7 years, such as diet, demographics, quality of living/social environment, and clinical records) were registered. We used multiple models to determine microbiome associations with pollutants including multi-determinant multivariate analysis of variance and linear correlations (wUnifrac, Bray-Curtis and Aitchison ß-diversity distances), single-pollutant permutational multivariate analysis of variance adjusting for co-variates (Aitchison), and multivariable association model with single taxa (MaAsLin2; genus). Sensitivity analysis was performed including genetic data in a subset of 107 children. Results: Active smoking in pregnancy was systematically associated with microbiome composition and ß-diversity (R2 2-4%, p < 0.05, Aitchison), independently of other co-determinants. However, in the adjusted single pollutant models (PERMANOVA), we did not find any significant association. An increased relative abundance of Dorea and decreased relative abundance of Akkermansia were associated with smoking during pregnancy (q < 0.05). Discussion: Our findings suggest a long-term sustainable effect of prenatal tobacco exposure on the children's gut microbiota. This effect was not found for mercury exposure or tobacco exposure during childhood. Assessing the role of these exposures on the children's microbiota, considering multiple environmental factors, should be further investigated.
• dc.description.sponsorship The author(s) declare financial support was received for the research, authorship, and/or publication of this article. The study has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 874583 (ATHLETE project). We acknowledge support from grants from Instituto de Salud Carlos III (Red INMA G03/176; CB06/02/0041; PI041436; PI081151 incl. FEDER funds, FIS/FEDER 19/1338), CIBERESP, Generalitat de Catalunya-CIRIT 1999SGR 00241, Generalitat de Catalunya-AGAUR 2009 SGR 501, Fundació La marató de TV3 (090430) and EU Commission (261357). We acknowledge support from the grant CEX2018-000806-S funded by MCIN/AEI/ 10.13039/501100011033, and support from the Generalitat de Catalunya through the CERCA Program. The study has also received funding from the CIAICO (2021/132), CPII20/00006, MS20/0006 and Alicia Koplowitz Foundation 2017, PI17/00663. L.M. is funded by a Juan de la Cierva-Incorporación fellowship (IJC2018-035394-I) awarded by the Spanish Ministerio de Economía, Industria y Competitividad.
• dc.format.mimetype application/pdf
• dc.identifier.citation Pérez-Castro S, D'Auria G, Llambrich M, Fernández-Barrés S, Lopez-Espinosa MJ, Llop S, et al. Influence of perinatal and childhood exposure to tobacco and mercury in children's gut microbiota. Front Microbiol. 2024 Jan 5;14:1258988. DOI: 10.3389/fmicb.2023.1258988
• dc.identifier.doi http://dx.doi.org/10.3389/fmicb.2023.1258988
• dc.identifier.issn 1664-302X
• dc.identifier.uri http://hdl.handle.net/10230/60605
• dc.language.iso eng
• dc.publisher Frontiers
• dc.relation.ispartof Front Microbiol. 2024 Jan 5;14:1258988
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/874583
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/261357
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/CEX2018-000806-S
• dc.rights © 2024 Pérez-Castro, D’Auria, Llambrich, Fernández-Barrés, Lopez-Espinosa, Llop, Regueiro, Bustamante, Francino, Vrijheid and Maitre. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. http://creativecommons.org/licenses/by/4.0/
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword 16S rRNA
• dc.subject.keyword Birth cohort
• dc.subject.keyword Children
• dc.subject.keyword Diet
• dc.subject.keyword Gut microbiota
• dc.subject.keyword Mercury
• dc.subject.keyword Second-hand smoke
• dc.subject.keyword Smoking during pregnancy
• dc.title Influence of perinatal and childhood exposure to tobacco and mercury in children's gut microbiota
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion