Mitochondrial Carriers Link the Catabolism of Hydroxyaromatic Compounds to the Central Metabolism in Candida parapsilosis

Zeman, Igor; Neboháčová, Martina; Gérecová, Gabriela; Katonová, Kornélia; Jánošíková, Eva; Jakúbková, Michaela; Centárová, Ivana; Dunčková, Ivana; Tomáška, L'ubomír; Pryszcz, Leszek Piotr, 1985-; Gabaldón Estevan, Juan Antonio, 1973-; Nosek, Jozef

Mitochondrial Carriers Link the Catabolism of Hydroxyaromatic Compounds to the Central Metabolism in Candida parapsilosis

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dc.contributor.author Zeman, Igorca
dc.contributor.author Neboháčová, Martinaca
dc.contributor.author Gérecová, Gabrielaca
dc.contributor.author Katonová, Kornéliaca
dc.contributor.author Jánošíková, Evaca
dc.contributor.author Jakúbková, Michaelaca
dc.contributor.author Centárová, Ivanaca
dc.contributor.author Dunčková, Ivanaca
dc.contributor.author Tomáška, L'ubomírca
dc.contributor.author Pryszcz, Leszek Piotr, 1985-ca
dc.contributor.author Gabaldón Estevan, Juan Antonio, 1973-ca
dc.contributor.author Nosek, Jozefca
dc.date.accessioned 2017-04-24T10:30:00Z
dc.date.available 2017-04-24T10:30:00Z
dc.date.issued 2016
dc.description.abstract The pathogenic yeast Candida parapsilosis metabolizes hydroxyderivatives of benzene and benzoic acid to compounds channeled into central metabolism, including the mitochondrially localized tricarboxylic acid cycle, via the 3-oxoadipate and gentisate pathways. The orchestration of both catabolic pathways with mitochondrial metabolism as well as their evolutionary origin is not fully understood. Our results show that the enzymes involved in these two pathways operate in the cytoplasm with the exception of the mitochondrially targeted 3-oxoadipate CoA-transferase (Osc1p) and 3-oxoadipyl-CoA thiolase (Oct1p) catalyzing the last two reactions of the 3-oxoadipate pathway. The cellular localization of the enzymes indicates that degradation of hydroxyaromatic compounds requires a shuttling of intermediates, cofactors, and products of the corresponding biochemical reactions between cytosol and mitochondria. Indeed, we found that yeast cells assimilating hydroxybenzoates increase the expression of genes SFC1, LEU5, YHM2, and MPC1 coding for succinate/fumarate carrier, coenzyme A carrier, oxoglutarate/citrate carrier, and the subunit of pyruvate carrier, respectively. A phylogenetic analysis uncovered distinct evolutionary trajectories for sparsely distributed gene clusters coding for enzymes of both pathways. Whereas the 3-oxoadipate pathway appears to have evolved by vertical descent combined with multiple losses, the gentisate pathway shows a striking pattern suggestive of horizontal gene transfer to the evolutionarily distant Mucorales.
dc.description.sponsorship This work was supported by the Slovak grant agency (VEGA) (1/0333/15 and 1/0052/16) and the Slovak Research and Development Agency (APVV) (14-0253 and 15-0022). The T.G. group is supported in part by a grant from the Spanish ministry of Economy and Competitiveness (BIO2012-37161), a grant from the Qatar National Research Fund (NPRP 5-298-3-086), and a grant from the European Research Council (ERC) under the European Union’s Seventh Framework Program (FP/2007-2013)/ERC (grant agreement no. ERC-2012-StG-310325).
dc.format.mimetype application/pdfca
dc.identifier.citation Zeman I, Neboháčová M, Gérecová G, Katonová K, Jánošíková E, Jakúbková M et al. Mitochondrial Carriers Link the Catabolism of Hydroxyaromatic Compounds to the Central Metabolism in Candida parapsilosis. G3-Genes Genomes Genetics. 2016;6(12):4047-58. DOI: 10.1534/g3.116.034389
dc.identifier.doi http://dx.doi.org/10.1534/g3.116.034389
dc.identifier.issn 2160-1836
dc.identifier.uri http://hdl.handle.net/10230/30886
dc.language.iso eng
dc.publisher Genetics Society of Americaca
dc.relation.ispartof G3-Genes Genomes Genetics. 2016;6(12):4047-58
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/310325
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2012-37161
dc.rights © Genetics Society of America. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject.keyword Gentisate pathway
dc.subject.keyword 3-Oxoadipate pathway
dc.subject.keyword Catabolism of hydroxybenzoates
dc.subject.keyword Mitochondrial carrier
dc.subject.keyword Evolution of biochemical pathways
dc.title Mitochondrial Carriers Link the Catabolism of Hydroxyaromatic Compounds to the Central Metabolism in Candida parapsilosisca
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/publishedVersion

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