ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

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• dc.contributor.author García-Castro, Helena
• dc.contributor.author Kenny, Nathan J.
• dc.contributor.author Iglesias, Marta
• dc.contributor.author Álvarez Campos, Patricia
• dc.contributor.author Mason, Vincent
• dc.contributor.author Elek, Anamaria
• dc.contributor.author Schönauer, Anna
• dc.contributor.author Sleight, Victoria A.
• dc.contributor.author Neiro, Jakke
• dc.contributor.author Aboobaker, Aziz
• dc.contributor.author Permanyer, Jon
• dc.contributor.author Irimia Martínez, Manuel
• dc.contributor.author Sebé-Pedrós, Arnau
• dc.contributor.author Solana, Jordi
• dc.date.accessioned 2021-05-19T11:24:48Z
• dc.date.available 2021-05-19T11:24:48Z
• dc.date.issued 2021
• dc.description.abstract Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are sortable and permeable. As a proof of principle, we provide single-cell transcriptomic data of different species, using both droplet-based and combinatorial barcoding single-cell methods. ACME uses affordable reagents, can be done in most laboratories and even in the field, and thus will accelerate our knowledge of cell types across the tree of life.
• dc.description.sponsorship This work was supported by an MRC grant (MR/S007849/1) and a Royal Society Grant (RGS\R1\191278) to JS. HG-C was supported by a Nigel Groome studentship from Oxford Brookes University. PA-C was supported by an EMBO Long Term Fellowship (ALTF-217-2018). JN was supported by funding from a BBSRC grant (BB/M011224/1) and the Osk. Huttunen Foundation (Doctoral grant)
• dc.format.mimetype application/pdf
• dc.identifier.citation García-Castro H, Kenny NJ, Iglesias M, Álvarez-Campos P, Mason V, Elek A et al. ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics. Genome Biol. 2021 Apr 8;22(1):89. DOI: 10.1186/s13059-021-02302-5
• dc.identifier.doi http://dx.doi.org/10.1186/s13059-021-02302-5
• dc.identifier.issn 1474-7596
• dc.identifier.uri http://hdl.handle.net/10230/47612
• dc.language.iso eng
• dc.publisher BioMed Central
• dc.rights © Helena García-Castro et al 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.subject.other Genètica
• dc.title ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion