Unravelling the consequences of the bacteriophages in human samples

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• dc.contributor.author Blanco Picazo, Pedro
• dc.contributor.author Fernández Orth, Dietmar
• dc.contributor.author Brown Jaque, Maryury
• dc.contributor.author Miró, Elisenda
• dc.contributor.author Espinal, Paula
• dc.contributor.author Rodríguez Rubio, Lorena
• dc.contributor.author Muniesa, Maite
• dc.contributor.author Navarro, Ferran
• dc.date.accessioned 2020-05-14T06:48:55Z
• dc.date.available 2020-05-14T06:48:55Z
• dc.date.issued 2020
• dc.description.abstract Bacteriophages are abundant in human biomes and therefore in human clinical samples. Although this is usually not considered, they might interfere with the recovery of bacterial pathogens at two levels: 1) by propagating in the enrichment cultures used to isolate the infectious agent, causing the lysis of the bacterial host and 2) by the detection of bacterial genes inside the phage capsids that mislead the presence of the bacterial pathogen. To unravel these interferences, human samples (n = 271) were analyzed and infectious phages were observed in 11% of blood culture, 28% of serum, 45% of ascitic fluid, 14% of cerebrospinal fluid and 23% of urine samples. The genetic content of phage particles from a pool of urine and ascitic fluid samples corresponded to bacteriophages infecting different bacterial genera. In addition, many bacterial genes packaged in the phage capsids, including antibiotic resistance genes and 16S rRNA genes, were detected in the viromes. Phage interference can be minimized applying a simple procedure that reduced the content of phages up to 3 logs while maintaining the bacterial load. This method reduced the detection of phage genes avoiding the interference with molecular detection of bacteria and reduced the phage propagation in the cultures, enhancing the recovery of bacteria up to 6 logs.
• dc.format.mimetype application/pdf
• dc.identifier.citation Blanco-Picazo P, Fernández-Orth D, Brown-Jaque M, Miró E, Espinal P, Rodríguez-Rubio L, Muniesa M, Navarro F. Unravelling the consequences of the bacteriophages in human samples. Sci Rep. 2020; 10(1):6737. DOI: 10.1038/s41598-020-63432-7
• dc.identifier.doi http://dx.doi.org/10.1038/s41598-020-63432-7
• dc.identifier.issn 2045-2322
• dc.identifier.uri http://hdl.handle.net/10230/44536
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof Sci Rep. 2020; 10(1):6737
• dc.rights © The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Bacterial infection
• dc.subject.keyword Bacteriophages
• dc.title Unravelling the consequences of the bacteriophages in human samples
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion