Developing a platform for secretion of biomolecules in Mycoplasma feriruminatoris
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- dc.contributor.author Gonzalez de Miguel, Javier
- dc.contributor.author Montero-Blay, Ariadna, 1994-
- dc.contributor.author Ciampi, Ludovica
- dc.contributor.author Rodriguez-Arce, Irene
- dc.contributor.author Serrano Pubull, Luis, 1982-
- dc.date.accessioned 2024-09-06T06:14:20Z
- dc.date.available 2024-09-06T06:14:20Z
- dc.date.issued 2024
- dc.description.abstract Background: Having a simple and fast dividing organism capable of producing and exposing at its surface or secreting functional complex biomolecules with disulphide bridges is of great interest. The mycoplasma bacterial genus offers a set of relevant properties that make it an interesting chassis for such purposes, the main one being the absence of a cell wall. However, due to their slow growth, they have rarely been considered as a potential platform in this respect. This notion may be challenged with the recent discovery of Mycoplasma feriruminatoris, a species with a dividing time close to that of common microbial workhorses. So far, no tools for heterologous protein expression nor secretion have been described for it. Results: The work presented here develops the fast-dividing M. feriruminatoris as a tool for secreting functional biomolecules of therapeutic interest that could be used for screening functional mutants as well as potentially for protein-protein interactions. Based on RNAseq, quantitative proteomics and promoter sequence comparison we have rationally designed optimal promoter sequences. Then, using in silico analysis, we have identified putative secretion signals that we validated using a luminescent reporter. The potential of the resulting secretion cassette has been shown with set of active clinically relevant proteins (interleukins and nanobodies). Conclusions: We have engineered Mycoplasma feriruminatoris for producing and secreting functional proteins of medical interest
- dc.format.mimetype application/pdf
- dc.identifier.citation Gonzalez-de-Miguel J, Montero-Blay A, Ciampi L, Rodriguez-Arce I, Serrano L. Developing a platform for secretion of biomolecules in Mycoplasma feriruminatoris. Microb Cell Fact. 2024 Apr 30;23(1):124. DOI: 10.1186/s12934-024-02392-3
- dc.identifier.doi http://dx.doi.org/10.1186/s12934-024-02392-3
- dc.identifier.issn 1475-2859
- dc.identifier.uri http://hdl.handle.net/10230/61029
- dc.language.iso eng
- dc.publisher BioMed Central
- dc.relation.ispartof Microb Cell Fact. 2024 Apr 30;23(1):124
- dc.rights © The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.other Biomolècules
- dc.title Developing a platform for secretion of biomolecules in Mycoplasma feriruminatoris
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion