Single-cell proteo-genomic reference maps of the hematopoietic system enable the purification and massive profiling of precisely defined cell states

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• dc.contributor.author Triana, Sergio
• dc.contributor.author Velten, Lars
• dc.contributor.author Haas, Simon
• dc.date.accessioned 2022-04-01T09:19:20Z
• dc.date.available 2022-04-01T09:19:20Z
• dc.date.issued 2021
• dc.description.abstract Single-cell genomics technology has transformed our understanding of complex cellular systems. However, excessive cost and a lack of strategies for the purification of newly identified cell types impede their functional characterization and large-scale profiling. Here, we have generated high-content single-cell proteo-genomic reference maps of human blood and bone marrow that quantitatively link the expression of up to 197 surface markers to cellular identities and biological processes across all main hematopoietic cell types in healthy aging and leukemia. These reference maps enable the automatic design of cost-effective high-throughput cytometry schemes that outperform state-of-the-art approaches, accurately reflect complex topologies of cellular systems and permit the purification of precisely defined cell states. The systematic integration of cytometry and proteo-genomic data enables the functional capacities of precisely mapped cell states to be measured at the single-cell level. Our study serves as an accessible resource and paves the way for a data-driven era in cytometry.
• dc.description.sponsorship This work was supported financially by the Emerson foundation grant 643577 (to L.V.), grant PID2019-108082GA-I00 from the Spanish Ministry of Science, Innovation and Universities (MCIU/AEI/FEDER, UE), the German Bundesministerium für Bildung und Forschung (BMBF) through the Juniorverbund in der Systemmedizin ‘LeukoSyStem’ (FKZ 01ZX1911D to L.V., S.H. and S.R.), SFB873, FOR2674 and FOR2033 funded by the Deutsche Forschungsgemeinschaft (DFG), the ERC Consolidator Grant METACELL (773089) (to T.A.) and the José Carreras Foundation for Leukemia Research (grant no. DCJLS 20 R/2017 to L.V., A.T. and S.H.). L.V. acknowledges the support of the Spanish Ministry of Science and Innovation to the EMBL partnership, the Centro de Excelencia Severo Ochoa and ‘the CERCA Programme/Generalitat de Catalunya
• dc.format.mimetype application/pdf
• dc.identifier.citation Triana S, Vonficht D, Jopp-Saile L, Raffel S, Lutz R, Leonce D et al. Single-cell proteo-genomic reference maps of the hematopoietic system enable the purification and massive profiling of precisely defined cell states. Nat Immunol. 2021 Dec;22(12):1577-89. DOI: 10.1038/s41590-021-01059-0
• dc.identifier.doi http://dx.doi.org/10.1038/s41590-021-01059-0
• dc.identifier.issn 1529-2908
• dc.identifier.uri http://hdl.handle.net/10230/52811
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/773089
• dc.rights © Sergio Triana et al. 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.subject.other Genètica
• dc.subject.other Genòmica
• dc.subject.other Proteòmica
• dc.subject.other Leucèmia
• dc.title Single-cell proteo-genomic reference maps of the hematopoietic system enable the purification and massive profiling of precisely defined cell states
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion