Expression profiling of microRNAs in human bone tissue from postmenopausal women

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• dc.contributor.author Ugarte Corbalán, Laura de, 1988-ca
• dc.contributor.author Serra-Vinardell, Jennyca
• dc.contributor.author Nonell Mazelon, Lara, 1972-ca
• dc.contributor.author Balcells, Susanaca
• dc.contributor.author Arnal, Magdalenaca
• dc.contributor.author Nogués Solan, Francesc Xavierca
• dc.contributor.author Mellibovsky, Leonardoca
• dc.contributor.author Grinberg, Danielca
• dc.contributor.author Díez Pérez, Adolfoca
• dc.contributor.author Garcia Giralt, Natàliaca
• dc.date.accessioned 2018-06-04T07:42:32Z
• dc.date.issued 2018
• dc.description.abstract Bone tissue is composed of several cell types, which express their own microRNAs (miRNAs) that will play a role in cell function. The set of total miRNAs expressed in all cell types configures the specific signature of the bone tissue in one physiological condition. The aim of this study was to explore the miRNA expression profile of bone tissue from postmenopausal women. Tissue was obtained from trabecular bone and was analyzed in fresh conditions (n = 6). Primary osteoblasts were also obtained from trabecular bone (n = 4) and human osteoclasts were obtained from monocyte precursors after in vitro differentiation (n = 5). MicroRNA expression profiling was obtained for each sample by microarray and a global miRNA analysis was performed combining the data acquired in all the microarray experiments. From the 641 miRNAs detected in bone tissue samples, 346 (54%) were present in osteoblasts and/or osteoclasts. The other 46% were not identified in any of the bone cells analyzed. Intersection of osteoblast and osteoclast arrays identified 101 miRNAs shared by both cell types, which accounts for 30-40% of miRNAs detected in these cells. In osteoblasts, 266 miRNAs were detected, of which 243 (91%) were also present in the total bone array, representing 38% of all bone miRNAs. In osteoclasts, 340 miRNAs were detected, of which 196 (58%) were also present in the bone tissue array, representing 31% of all miRNAs detected in total bone. These analyses provide an overview of miRNAs expressed in bone tissue, broadening our knowledge in the microRNA field.
• dc.description.sponsorship This work was supported by the Red Temática de Investigación Cooperativa en Envejecimiento y Fragilidad (RETICEF; RD12/0043/0022) and Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable (CIBERFES; CB16/10/00245), and the Grants PI10/01537, PI13/00116, and PI13/00444 from FIS (Carlos III Health Institute, Science and Innovation Ministry); SAF2016-75948-R, from Ministerio de Economia y Competitividad, and 2014SGR-932 from Generalitat de Catalunya. FEDER funds also supported this study.
• dc.format.mimetype application/pdf
• dc.identifier.citation De-Ugarte L, Serra-Vinardell J, Nonell L, Balcells S, Arnal M, Nogues X. et al. Expression profiling of microRNAs in human bone tissue from postmenopausal women. Hum Cell. 2018 Jan;31(1):33-41. DOI: 10.1007/s13577-017-0181-y
• dc.identifier.doi http://dx.doi.org/10.1007/s13577-017-0181-y
• dc.identifier.issn 0914-7470
• dc.identifier.uri http://hdl.handle.net/10230/34793
• dc.language.iso eng
• dc.publisher Springerca
• dc.relation.ispartof Human Cell. 2018 Jan;31(1):33-41
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2016-75948-R
• dc.rights © Springer The final publication is available at Springer via http://dx.doi.org/10.1007/s13577-017-0181-y.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.keyword Bone tissue
• dc.subject.keyword MicroRNA
• dc.subject.keyword Microarrays
• dc.subject.keyword Osteoblast
• dc.subject.keyword Osteoclast
• dc.subject.other Ossos -- Fisiologia
• dc.subject.other Menopausa -- Aspectes psicològics
• dc.title Expression profiling of microRNAs in human bone tissue from postmenopausal womenca
• dc.type info:eu-repo/semantics/book
• dc.type.version info:eu-repo/semantics/acceptedVersion