Phylogenetic and functional characterization of water bears (Tardigrada) tubulins

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• dc.contributor.author Novotná Floriančičová, Kamila
• dc.contributor.author Baltzis, Athanasios
• dc.contributor.author Smejkal, Jiří
• dc.contributor.author Czerneková, Michaela
• dc.contributor.author Kaczmarek, Łukasz
• dc.contributor.author Malý, Jan
• dc.contributor.author Notredame, Cedric
• dc.contributor.author Vinopal, Stanislav
• dc.date.accessioned 2023-06-12T05:46:18Z
• dc.date.available 2023-06-12T05:46:18Z
• dc.date.issued 2023
• dc.description.abstract Tardigrades are microscopic ecdysozoans that can withstand extreme environmental conditions. Several tardigrade species undergo reversible morphological transformations and enter into cryptobiosis, which helps them to survive periods of unfavorable environmental conditions. However, the underlying molecular mechanisms of cryptobiosis are mostly unknown. Tubulins are evolutionarily conserved components of the microtubule cytoskeleton that are crucial in many cellular processes. We hypothesize that microtubules are necessary for the morphological changes associated with successful cryptobiosis. The molecular composition of the microtubule cytoskeleton in tardigrades is unknown. Therefore, we analyzed and characterized tardigrade tubulins and identified 79 tardigrade tubulin sequences in eight taxa. We found three α-, seven β-, one γ-, and one ε-tubulin isoform. To verify in silico identified tardigrade tubulins, we also isolated and sequenced nine out of ten predicted Hypsibius exemplaris tubulins. All tardigrade tubulins were localized as expected when overexpressed in mammalian cultured cells: to the microtubules or to the centrosomes. The presence of a functional ε-tubulin, clearly localized to centrioles, is attractive from a phylogenetic point of view. Although the phylogenetically close Nematoda lost their δ- and ε-tubulins, some groups of Arthropoda still possess them. Thus, our data support the current placement of tardigrades into the Panarthropoda clade.
• dc.format.mimetype application/pdf
• dc.identifier.citation Novotná K, Baltzis A, Smejkal J, Czerneková M, Kaczmarek Ł, Malý J, et al. Phylogenetic and functional characterization of water bears (Tardigrada) tubulins. Sci Rep. 2023 Mar 30;13:5194. DOI: 10.1038/s41598-023-31992-z
• dc.identifier.doi http://dx.doi.org/10.1038/s41598-023-31992-z
• dc.identifier.issn 2045-2322
• dc.identifier.uri http://hdl.handle.net/10230/57152
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof Scientific Reports . 2023 Mar 30;13:5194
• dc.rights This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.other Filogènia
• dc.subject.other Ecdisona
• dc.subject.other Microorganismes
• dc.subject.other Microbiologia
• dc.title Phylogenetic and functional characterization of water bears (Tardigrada) tubulins
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion