The changing chromatome as a driver of disease: a panoramic view from different methodologies

Espejo, Isabel; Di Croce, Luciano; Aranda Aragón, Sergio

doi:http://dx.doi.org/10.1002/bies.202000203

The changing chromatome as a driver of disease: a panoramic view from different methodologies

Citation

Espejo I, Di Croce L, Aranda S. The changing chromatome as a driver of disease: a panoramic view from different methodologies. Bioessays. 2020 Dec; 42(12):e 2000203. DOI: 10.1002/bies.202000203

Abstract

Chromatin-bound proteins underlie several fundamental cellular functions, such as control of gene expression and the faithful transmission of genetic and epigenetic information. Components of the chromatin proteome (the "chromatome") are essential in human life, and mutations in chromatin-bound proteins are frequently drivers of human diseases, such as cancer. Proteomic characterization of chromatin and de novo identification of chromatin interactors could, thus, reveal important and perhaps unexpected players implicated in human physiology and disease. Recently, intensive research efforts have focused on developing strategies to characterize the chromatome composition. In this review, we provide an overview of the dynamic composition of the chromatome, highlight the importance of its alterations as a driving force in human disease (and particularly in cancer), and discuss the different approaches to systematically characterize the chromatin-bound proteome in a global manner.