The translational landscape of the splicing factor SRSF1 and its role in mitosis

Maslon, Magdalena M.; Heras, Sara R.; Bellora Pereyra, Nicolás; Eyras Jiménez, Eduardo; Cáceres, Javier F.

The translational landscape of the splicing factor SRSF1 and its role in mitosis

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dc.contributor.author Maslon, Magdalena M.ca
dc.contributor.author Heras, Sara R.ca
dc.contributor.author Bellora Pereyra, Nicolásca
dc.contributor.author Eyras Jiménez, Eduardoca
dc.contributor.author Cáceres, Javier F.ca
dc.date.accessioned 2015-06-04T06:57:00Z
dc.date.available 2015-06-04T06:57:00Z
dc.date.issued 2014ca
dc.description.abstract The shuttling serine/arginine rich (SR) protein SRSF1 (previously known as SF2/ASF) is a splicing regulator that also activates translation in the cytoplasm. In order to dissect the gene network that is translationally regulated by SRSF1, we performed a high-throughput deep sequencing analysis of polysomal fractions in cells overexpressing SRSF1. We identified approximately 1500 mRNAs that are translational targets of SRSF1. These include mRNAs encoding proteins involved in cell cycle regulation, such as spindle, kinetochore, and M phase proteins, which are essential for accurate chromosome segregation. Indeed, we show that translational activity of SRSF1 is required for normal mitotic progression. Furthermore, we found that mRNAs that display alternative splicing changes upon SRSF1 overexpression are also its translational targets, strongly suggesting that SRSF1 couples pre-mRNA splicing and translation. These data provide insights on the complex role of SRSF1 in the control of gene expression at multiple levels and its implications in cancer.en
dc.description.sponsorship MRC Core Funding Sara R Heras, Javier F Cáceres. Wellcome Trust Senior Investigator Award Grant 095518/Z/11/Z Magdalena M Maslon, Javier F Cáceres. Marie Curie IntraEuropean fellowship Sara R Heras. Consolider RNAReg CSD2009-00080 Nicolas Bellora, Eduardo Eyras. Ministerio de Economia y Competitividad BIO2011-23920 Nicolas Bellora, Eduardo Eyras. Sandra Ibarra Foundation FSI2011-35 Nicolas Bellora, Eduardo Eyras.
dc.format.mimetype application/pdfca
dc.identifier.citation Maslon MM, Heras SR, Bellora N, Eyras E, Caceres JF. The translational landscape of the splicing factor SRSF1 and its role in mitosis. eLIFE. 2014; 3: e02028. DOI 10.7554/eLife.02028ca
dc.identifier.doi http://dx.doi.org/10.7554/eLife.02028
dc.identifier.issn 2050-084Xca
dc.identifier.uri http://hdl.handle.net/10230/23729
dc.language.iso engca
dc.publisher eLifeca
dc.relation.ispartof eLIFE. 2014;3:e02028
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/CSD2009-00080
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2011-23920
dc.rights © Copyright Maslon et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.en
dc.rights.accessRights info:eu-repo/semantics/openAccessca
dc.rights.uri https://creativecommons.org/licenses/by/3.0/
dc.subject.other Seqüència de nucleòtids -- Informàticaca
dc.subject.other Regulació genètica -- Informàticaca
dc.title The translational landscape of the splicing factor SRSF1 and its role in mitosisen
dc.type info:eu-repo/semantics/articleca
dc.type.version info:eu-repo/semantics/publishedVersionca

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