Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle

Soria, Ivana; Quattrocchi, Valeria; Langellotti, Cecilia; Gammella, Mariela; Digiacomo Sebastian; Garcia de la Torre, Beatriz; Andreu Martínez, David; Montoya, Maria; Sobrino, Francisco; Blanco, Esther; Zamorano, Patricia

Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle

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dc.contributor.author Soria, Ivanaca
dc.contributor.author Quattrocchi, Valeriaca
dc.contributor.author Langellotti, Ceciliaca
dc.contributor.author Gammella, Marielaca
dc.contributor.author Digiacomo Sebastianca
dc.contributor.author Garcia de la Torre, Beatrizca
dc.contributor.author Andreu Martínez, Davidca
dc.contributor.author Montoya, Mariaca
dc.contributor.author Sobrino, Franciscoca
dc.contributor.author Blanco, Estherca
dc.contributor.author Zamorano, Patriciaca
dc.date.accessioned 2017-11-14T09:21:26Z
dc.date.available 2017-11-14T09:21:26Z
dc.date.issued 2017
dc.description.abstract Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.
dc.description.sponsorship Work at INTA was supported by the National Institute of Agricultural Technology (grant number PNSA 1115052) and cooperation agreement INTA-INIA (Argentina-Spain SA 20938). Evaluation of vaccine immunogenicity and protection in cattle. Work at Universitat Pompeu Fabra and CBMSO was supported by MINECO, Spain (grant number AGL2014-52395-C2).
dc.format.mimetype application/pdfca
dc.identifier.citation Soria I, Quattrocchi V, Langellotti C, Gammella M, Digiacomo S, Garcia de la Torre B [et al]. Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle. PLoS One. 2017 Sep 26;12(9):e0185184. DOI: 10.1371/journal.pone.0185184
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0185184
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10230/33213
dc.language.iso eng
dc.publisher Public Library of Science (PLoS)ca
dc.relation.ispartof PLoS One. 2017 Sep 26;12(9):e0185184
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/AGL2014-52395-C2
dc.rights © 2017 Soria et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject.keyword Foot-and-mouth disease
dc.subject.keyword Dendrimers
dc.subject.keyword Peptides
dc.title Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattleca
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/publishedVersion

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