Golgi enzymes do not cycle through the endoplasmic reticulum during protein secretion or mitosis

Mostra el registre complet Registre parcial de l'ítem

  • dc.contributor.author Villeneuvea, Julien
  • dc.contributor.author Duran, Joan
  • dc.contributor.author Scarpa, Margherita
  • dc.contributor.author Bassaganyas Bars, Laia, 1985-
  • dc.contributor.author van Galen, Josse
  • dc.contributor.author Malhotra, Vivek
  • dc.date.accessioned 2025-01-21T07:39:08Z
  • dc.date.available 2025-01-21T07:39:08Z
  • dc.date.issued 2017
  • dc.description.abstract Golgi-specific sialyltransferase (ST) expressed as a chimera with the rapamycin-binding domain of mTOR, FRB, relocates to the endoplasmic reticulum (ER) in cells exposed to rapamycin that also express invariant chain (Ii)-FKBP in the ER. This result has been taken to indicate that Golgi-resident enzymes cycle to the ER constitutively. We show that ST-FRB is trapped in the ER even without Ii-FKBP upon rapamycin addition. This is because ER-Golgi–cycling FKBP proteins contain a C-terminal KDEL-like sequence, bind ST-FRB in the Golgi, and are transported together back to the ER by KDEL receptor–mediated retrograde transport. Moreover, depletion of KDEL receptor prevents trapping of ST-FRB in the ER by rapamycin. Thus ST-FRB cycles artificially by binding to FKBP domain–containing proteins. In addition, Golgi-specific O-linked glycosylation of a resident ER protein occurs only upon artificial fusion of Golgi membranes with ER. Together these findings support the consensus view that there is no appreciable mixing of Golgi-resident enzymes with ER under normal conditions.en
  • dc.description.sponsorship We thank all members of the Malhotra laboratory for valuable discussions and Arrate Mallabiabarrena and Raquel García for help with fluorescence microscopy. J.V. acknowledges support from a Marie Curie International Outgoing Fellowship within the 7th European Community Framework Program. V.M. is an Institució Catalana de Recerca i Estudis Avançats Professor at the Center for Genomic Regulation, and the work in his laboratory is funded by grants from Plan Nacional (BFU2008-00414), Consolider (CSD2009-00016), the Agència de Gestió d’Ajuts Universitaris i de Recerca Grups de Recerca Emergents (SGR2009-1488; AGAUR-Catalan Government), and the European Research Council (268692). The project received research funding from the European Union. We acknowledge support of the Spanish Ministry of Economy and Competitiveness, Centro de Excelencia Severo Ochoa 2013-2017. This article reflects the views of only the authors. The European Union is not liable for any use that may be made of the information contained herein.en
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Villeneuve J, Duran J, Scarpa M, Bassaganyas L, Van Galen J, Malhotra V. Golgi enzymes do not cycle through the endoplasmic reticulum during protein secretion or mitosis. MBoC. 2017 Jan;28(1):141-51. DOI: 10.1091/mbc.e16-08-0560
  • dc.identifier.doi http://dx.doi.org/10.1091/mbc.e16-08-0560
  • dc.identifier.issn 1939-4586
  • dc.identifier.uri http://hdl.handle.net/10230/69214
  • dc.language.iso eng
  • dc.publisher American Society for Cell Biology
  • dc.relation.ispartof Molecular Biology of the Cell. 2017 Jan;28(1):141-51
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/268692
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2008-00414
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/CSD2009-00016
  • dc.rights © 2017 Villeneuve, Duran, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0
  • dc.subject.other Aparell de Golgica
  • dc.subject.other Reticle endoplasmàticca
  • dc.subject.other Enzimsca
  • dc.title Golgi enzymes do not cycle through the endoplasmic reticulum during protein secretion or mitosisen
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

Col·leccions

Articles (Center for Genomic Regulation (CRG))