Non-canonical role for the BAF complex subunit DPF3 in mitosis and ciliogenesis

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• dc.contributor.author Verrillo, Giulia
• dc.contributor.author Obeid, Anna Maria
• dc.contributor.author Genco, Alexia
• dc.contributor.author Scrofani, Jacopo, 1984-
• dc.contributor.author Orange, François
• dc.contributor.author Hanache, Sarah
• dc.contributor.author Mignon, Julien
• dc.contributor.author Leyder, Tanguy
• dc.contributor.author Michaux, Catherine
• dc.contributor.author Kempeneers, Céline
• dc.contributor.author Bricmont, Noëmie
• dc.contributor.author Herkenne, Stephanie
• dc.contributor.author Vernos, Isabelle, 1959-
• dc.contributor.author Martin, Maud
• dc.contributor.author Mottet, Denis
• dc.date.accessioned 2024-09-04T07:16:14Z
• dc.date.available 2024-09-04T07:16:14Z
• dc.date.issued 2024
• dc.description.abstract DPF3, along with other subunits, is a well-known component of the BAF chromatin remodeling complex, which plays a key role in regulating chromatin remodeling activity and gene expression. Here, we elucidated a non-canonical localization and role for DPF3. We showed that DPF3 dynamically localizes to the centriolar satellites in interphase and to the centrosome, spindle midzone and bridging fiber area, and midbodies during mitosis. Loss of DPF3 causes kinetochore fiber instability, unstable kinetochore-microtubule attachment and defects in chromosome alignment, resulting in altered mitotic progression, cell death and genomic instability. In addition, we also demonstrated that DPF3 localizes to centriolar satellites at the base of primary cilia and is required for ciliogenesis by regulating axoneme extension. Taken together, these findings uncover a moonlighting dual function for DPF3 during mitosis and ciliogenesis.
• dc.description.sponsorship This work was supported by grants from the Fonds De La Recherche Scientifique – FNRS, Belgium, Télévie, Fonds Léon Fredericq at the Université de Liège, and Fonds Spéciaux de Recherche of the Université de Liège. Work in the Vernos laboratory was supported by grants from Ministerio de Economía y Competitividad (BFU2015-68726-P), Ministerio de Ciencia, Innovación y Universidades (PGC2018-096976-B-I00) and Generalitat de Catalunya (AGAUR-SGR 478). Open access funding provided by Fonds Léon Fredericq at the Université de Liège. Deposited in PMC for immediate release.
• dc.format.mimetype application/pdf
• dc.identifier.citation Verrillo G, Obeid AM, Genco A, Scrofani J, Orange F, Hanache S, et al. Non-canonical role for the BAF complex subunit DPF3 in mitosis and ciliogenesis. J Cell Sci. 2024 May 1;137(9):jcs261744. DOI: 10.1242/jcs.261744
• dc.identifier.doi http://dx.doi.org/10.1242/jcs.261744
• dc.identifier.issn 0021-9533
• dc.identifier.uri http://hdl.handle.net/10230/61000
• dc.language.iso eng
• dc.publisher Company of Biologists
• dc.relation.ispartof J Cell Sci. 2024 May 1;137(9):jcs261744
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-68726-P
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-096976-B-I00
• dc.rights © 2024. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0
• dc.subject.keyword Bridging fiber
• dc.subject.keyword Centriolar satellites
• dc.subject.keyword Chromosome alignment
• dc.subject.keyword Ciliogenesis
• dc.subject.keyword DPF3
• dc.subject.keyword Genomic instability
• dc.subject.keyword Mitosis
• dc.title Non-canonical role for the BAF complex subunit DPF3 in mitosis and ciliogenesis
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion