Analysis of a long-term outbreak of XDR Pseudomonas aeruginosa: a molecular epidemiological study
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- dc.contributor.author Willmann, Matthiasca
- dc.contributor.author Bezdan, Danielaca
- dc.contributor.author Zapata Ortiz, Luis, 1985-ca
- dc.contributor.author Sušak, Hana, 1985-ca
- dc.contributor.author Vogel, Wichardca
- dc.contributor.author Schröppel, Klausca
- dc.contributor.author Liese, Janca
- dc.contributor.author Weidenmaier, Christopherca
- dc.contributor.author Autenrieth, Ingo B.ca
- dc.contributor.author Ossowski, Stephanca
- dc.contributor.author Peter, Silkeca
- dc.date.accessioned 2016-07-22T14:02:12Z
- dc.date.available 2016-07-22T14:02:12Z
- dc.date.issued 2015
- dc.description.abstract OBJECTIVES: Here we report on a long-term outbreak from 2009 to 2012 with an XDR Pseudomonas aeruginosa on two wards at a university hospital in southern Germany. METHODS: Whole-genome sequencing was performed on the outbreak isolates and a core genome was constructed for molecular epidemiological analysis. We applied a time-place-sequence algorithm to improve estimation of transmission probabilities. RESULTS: By using conventional infection control methods we identified 49 P. aeruginosa strains, including eight environmental isolates that belonged to ST308 (by MLST) and carried the metallo-β-lactamase IMP-8. Phylogenetic analysis on the basis of a non-recombinant core genome that contained 22 outbreak-specific SNPs revealed a pattern of four dominant clades with a strong phylogeographic structure and allowed us to determine the potential temporal origin of the outbreak to July 2008, 1 year before the index case was diagnosed. Superspreaders at the root of clades exhibited a high number of probable and predicted transmissions, indicating their exceptional position in the outbreak. CONCLUSIONS: Our results suggest that the initial expansion of dominant sublineages was driven by a few superspreaders, while environmental contamination seemed to sustain the outbreak for a long period despite regular environmental control measures.ca
- dc.description.sponsorship This work was supported by a grant from ‘la Caixa’ to L.Z.
- dc.format.mimetype application/pdfca
- dc.identifier.citation Willmann M, Bezdan D, Zapata L, Susak H, Vogel W, Schröppel K et al. Analysis of a long-term outbreak of XDR Pseudomonas aeruginosa: a molecular epidemiological study. Journal of antimicrobial chemotherapy. 2015;70(5):1322-30. DOI: 10.1093/jac/dku546ca
- dc.identifier.doi http://dx.doi.org/10.1093/jac/dku546
- dc.identifier.issn 0305-7453
- dc.identifier.uri http://hdl.handle.net/10230/27110
- dc.language.iso engca
- dc.publisher Oxford University Pressca
- dc.relation.ispartof Journal of antimicrobial chemotherapy. 2015;70(5):1322-30
- dc.rights © Oxford University Press. This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of antimicrobial chemotherapy following peer review. The definitive publisher-authenticated version Willmann M, Bezdan D, Zapata L, Susak H, Vogel W, Schröppel K et al. Analysis of a long-term outbreak of XDR Pseudomonas aeruginosa: a molecular epidemiological study. Journal of antimicrobial chemotherapy. 2015; 70(5): 1322-1330. DOI 10.1093/jac/dku546 is available online at: http://jac.oxfordjournals.org/content/70/5/1322.longca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.subject.keyword IMP carbapenemase
- dc.subject.keyword Outbreak investigation
- dc.subject.keyword Transmission analysis
- dc.subject.keyword Whole-genome sequencing
- dc.subject.other Bacterisca
- dc.subject.other Epidemiologiaca
- dc.title Analysis of a long-term outbreak of XDR Pseudomonas aeruginosa: a molecular epidemiological studyca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/acceptedVersionca