Prolonged immobilization exacerbates the loss of muscle mass and function induced by cancer-associated cachexia through enhanced proteolysis in mice

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• dc.contributor.author Mañas García, Laura, 1991-
• dc.contributor.author Penedo Vázquez, Antonio
• dc.contributor.author López Postigo, Adrián
• dc.contributor.author Deschrevel, Jorieke
• dc.contributor.author Durán, Xavier
• dc.contributor.author Barreiro Portela, Esther
• dc.date.accessioned 2021-03-31T06:06:17Z
• dc.date.available 2021-03-31T06:06:17Z
• dc.date.issued 2020
• dc.description.abstract We hypothesized that in mice with lung cancer (LC)-induced cachexia, periods of immobilization of the hindlimb (7 and 15 days) may further aggravate the process of muscle mass loss and function. Mice were divided into seven groups (n = 10/group): (1) non-immobilized control mice, (2) 7-day unloaded mice (7-day I), (3) 15-day unloaded mice (15-day I), (4) 21-day LC-cachexia group (LC 21-days), (5) 30-day LC-cachexia group (LC 30-days), (6) 21-day LC-cachexia group besides 7 days of unloading (LC 21-days + 7-day I), (7) 30-day LC-cachexia group besides 15 days of unloading (LC 30-days + 15-day I). Physiological parameters, body weight, muscle and tumor weights, phenotype and morphometry, muscle damage (including troponin I), proteolytic and autophagy markers, and muscle regeneration markers were identified in gastrocnemius muscle. In LC-induced cachexia mice exposed to hindlimb unloading, gastrocnemius weight, limb strength, fast-twitch myofiber cross-sectional area, and muscle regeneration markers significantly decreased, while tumor weight and area, muscle damage (troponin), and proteolytic and autophagy markers increased. In gastrocnemius of cancer-cachectic mice exposed to unloading, severe muscle atrophy and impaired function was observed along with increased muscle proteolysis and autophagy, muscle damage, and impaired muscle regeneration.
• dc.format.mimetype application/pdf
• dc.identifier.citation Mañas-García L, Penedo-Vázquez A, López-Postigo A, Deschrevel J, Durán X, Barreiro E. Prolonged immobilization exacerbates the loss of muscle mass and function induced by cancer-associated cachexia through enhanced proteolysis in mice. Int J Mol Sci. 2020; 21(21):8167. DOI: 10.3390/ijms21218167
• dc.identifier.doi http://dx.doi.org/10.3390/ijms21218167
• dc.identifier.issn 1422-0067
• dc.identifier.uri http://hdl.handle.net/10230/46996
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Int J Mol Sci. 2020; 21(21):8167
• dc.rights © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Cancer cachexia model
• dc.subject.keyword Muscle damage
• dc.subject.keyword Muscle proteolysis and autophagy
• dc.subject.keyword Muscle regeneration
• dc.subject.keyword Muscle unloading model
• dc.title Prolonged immobilization exacerbates the loss of muscle mass and function induced by cancer-associated cachexia through enhanced proteolysis in mice
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion