The interplay between disordered regions in RNAs and proteins modulates interactions within stress granules and processing bodies
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- dc.contributor.author Vandelli, Andrea
- dc.contributor.author Cid Samper, Fernando, 1991-
- dc.contributor.author Torrent Burgas, Marc
- dc.contributor.author Sánchez de Groot, Natalia
- dc.contributor.author Tartaglia, Gian Gaetano
- dc.date.accessioned 2021-12-01T07:35:37Z
- dc.date.available 2021-12-01T07:35:37Z
- dc.date.issued 2022
- dc.description.abstract Condensation, or liquid-like phase separation, is a phenomenon indispensable for the spatiotemporal regulation of molecules within the cell. Recent studies indicate that the composition and molecular organization of phase-separated organelles such as Stress Granules (SGs) and Processing Bodies (PBs) are highly variable and dynamic. A dense contact network involving both RNAs and proteins controls the formation of SGs and PBs and an intricate molecular architecture, at present poorly understood, guarantees that these assemblies sense and adapt to different stresses and environmental changes. Here, we investigated the physico-chemical properties of SGs and PBs components and studied the architecture of their interaction networks. We found that proteins and RNAs establishing the largest amount of contacts in SGs and PBs have distinct properties and intrinsic disorder is enriched in all protein-RNA, protein-protein and RNA-RNA interaction networks. The increase of disorder in proteins is accompanied by an enrichment in single-stranded regions of RNA binding partners. Our results suggest that SGs and PBs quickly assemble and disassemble through dynamic contacts modulated by unfolded domains of their components.
- dc.description.sponsorship The research leading to these results has been supported by European Research Council [RIBOMYLOME_309545 and ASTRA_855923], the H2020 projects [IASIS_727658 and INFORE_825080] and the Spanish Ministry of Science and Innovation (RYC2019-026752-I and PID2020-117454RA-I00).
- dc.format.mimetype application/pdf
- dc.identifier.citation Vandelli A, Cid Samper F, Torrent Burgas M, Sanchez de Groot N, Tartaglia GG. The interplay between disordered regions in RNAs and proteins modulates interactions within stress granules and processing bodies. J Mol Biol. 2022;434(1):167159. DOI: 10.1016/j.jmb.2021.167159
- dc.identifier.doi http://dx.doi.org/10.1016/j.jmb.2021.167159
- dc.identifier.issn 0022-2836
- dc.identifier.uri http://hdl.handle.net/10230/49113
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof J Mol Biol. 2022;434(1):167159
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/309545
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/855923
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/727658
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/825070
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/RYC2019-026752-I
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-117454RA-I00
- dc.rights © 2021 The Authors. Published by Elsevier Ltd.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
- dc.subject.keyword RNA and proteins
- dc.subject.keyword Fuzzy interactions
- dc.subject.keyword Interaction networks
- dc.subject.keyword Liquid-liquid phase separation
- dc.subject.keyword Structural disorder
- dc.title The interplay between disordered regions in RNAs and proteins modulates interactions within stress granules and processing bodies
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion