A protocol describing the genetic correction of somatic human cells and subsequent generation of IPS cell
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- dc.contributor.author Rodríguez Pizà, Ignasica
- dc.contributor.author Raya Chamorro, Ángelca
- dc.contributor.author Navarro, Susannaca
- dc.contributor.author Izpisúa Belmonte, J. C.ca
- dc.contributor.author Bueren, Juanca
- dc.contributor.author Consiglio, Antonellaca
- dc.contributor.author Sánchez Danés, Adriana, 1984-ca
- dc.contributor.author Guenechea, Guillermoca
- dc.contributor.author Richaud Patin, Yvonneca
- dc.date.accessioned 2011-07-27T08:28:04Z
- dc.date.available 2011-07-27T08:28:04Z
- dc.date.issued 2010ca
- dc.description.abstract The generation of patient-specific induced pluripotent stem cells (iPSCPSCPSCs) offers unprecedented opportunities for modeling and treating human disease. In combination with gene therapy, the iPSCPSCPSC technology can be used to generate disease-free progenitor cells of potential interest for autologous cell therapy. We explain a protocol for the reproducible generation of genetically corrected iPSCPSCPSCs starting from the skin biopsies of Fanconi anemia patients using retroviral transduction with OCT4, SOX2 and KLF4. Before reprogramming, the fibroblasts and/or keratinocytes of the patients are genetically corrected with lentiviruses expressing FANCA. The same approach may be used for other diseases susceptible to gene therapy correction. Genetically corrected, characterized lines of patient-specific iPSCPSCPSCs can be obtained in 4–5 months.
- dc.format.mimetype application/pdfca
- dc.identifier.citation Raya Á, Rodríguez I, Navarro S, Richaud-Patin Y, Guenechea G, Sánchez-Danés A et al. A protocol describing the genetic correction of somatic human cells and subsequent generation of iPS cells. Nat Protoc. 2010;5:647-60. DOI 10.1038/nprot.2010.9
- dc.identifier.doi http://dx.doi.org/10.1038/nprot.2010.9
- dc.identifier.issn 1754-2189
- dc.identifier.uri http://hdl.handle.net/10230/12431
- dc.language.iso engca
- dc.publisher Nature Publishing Groupca
- dc.relation.ispartof Nature Protocols. 2010;5(4):647-60
- dc.rights © Nature Publishing Groupca
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.other Medicina regenerativa
- dc.subject.other Cél·lules mare embrionàries
- dc.title A protocol describing the genetic correction of somatic human cells and subsequent generation of IPS cellca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersion