Non-linear formation of EtG and FAEEs after controlled administration of low to moderate doses of ethanol

Pérez Mañá, Clara; Farré Albaladejo, Magí; Pastor, Antonio; Fonseca Casals, Francina, 1972-; Torrens, Marta; Menoyo Colomer, Esther; Pujadas Bastardes, Mitona; Frias, Silvia; Langohr, Klaus; Torre Fornell, Rafael de la

Non-linear formation of EtG and FAEEs after controlled administration of low to moderate doses of ethanol

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dc.contributor.author Pérez Mañá, Claraca
dc.contributor.author Farré Albaladejo, Magíca
dc.contributor.author Pastor, Antonioca
dc.contributor.author Fonseca Casals, Francina, 1972-ca
dc.contributor.author Torrens, Martaca
dc.contributor.author Menoyo Colomer, Estherca
dc.contributor.author Pujadas Bastardes, Mitonaca
dc.contributor.author Frias, Silviaca
dc.contributor.author Langohr, Klausca
dc.contributor.author Torre Fornell, Rafael de laca
dc.date.accessioned 2018-03-20T07:54:54Z
dc.date.issued 2017
dc.description.abstract AIMS: Ethyl glucuronide (EtG) and fatty acid ethyl esters (FAEEs) are non-oxidative metabolites of alcohol that can be detected in conventional and non-conventional biological matrices for longer periods than alcohol. The aim was to describe the time courses of both biomarkers after ingestion of acute low-moderate doses of ethanol. METHODS: The study design was double-blind, randomized, crossover and controlled with placebo. Participants were distributed in three different cohorts: (a) Cohort-1: two doses of 18 and 30 g of ethanol and placebo were administered to 12 subjects; (b) Cohort-2: two doses of 6 and 12 g of ethanol and placebo were administered to six subjects and (c) Cohort-3: two doses of 24 and 42 g of ethanol and placebo were administered to six subjects. Each participant received two doses of ethanol and placebo. Plasma concentrations (0-6 h) of ethanol and specific FAEEs (palmitic, stearic, linoleic and oleic acid ethyl esters) and urinary concentrations of EtG (0-24 h) were measured. RESULTS: A dose-dependent increase in blood ethanol concentrations was observed. EtG excretion and FAEEs plasmatic concentrations showed a disproportionate increase with the ethanol dose suggesting non-linearity. Area under the curve (AUC0-6h) of ethanol concentrations showed a linear trend with non-oxidative metabolites' concentrations. CONCLUSION: The formation rate of ethanol non-oxidative biomarkers does not follow a linear trend, explained mainly by a disproportionate increase in AUC0-6h of ethanol concentrations in relation to dose. This observation should be taken into account when interpreting results in biological matrices in clinical and forensic settings. SHORT SUMMARY: A double-blind, randomized, crossover and controlled study was conducted administering ethanol (6-42 g). Ethyl glucuronide (EtG) excretion and fatty acid ethyl esters (FAEEs) plasmatic concentrations showed a disproportionate increase with the ethanol dose suggesting non-linearity. This observation should be taken into account when interpreting biomarker concentrations in clinical settings.
dc.format.mimetype application/pdf
dc.identifier.citation Pérez-Mañá C, Farré M, Pastor A, Fonseca F, Torrens M, Menoyo E et al. Non-Linear Formation of EtG and FAEEs after Controlled Administration of Low to Moderate Doses of Ethanol. Alcohol Alcohol. 2017 Sep 1;52(5):587-94. DOI: 10.1093/alcalc/agx033
dc.identifier.doi http://dx.doi.org/10.1093/alcalc/agx033
dc.identifier.issn 0735-0414
dc.identifier.uri http://hdl.handle.net/10230/34215
dc.language.iso eng
dc.publisher Oxford University Pressca
dc.relation.ispartof Alcohol Alcohol. 2017 Sep 1;52(5):587-94
dc.rights © Oxford University Press. This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Alcohol and alcoholism following peer review. The definitive publisher-authenticated version Pérez-Mañá C, Farré M, Pastor A, Fonseca F, Torrens M, Menoyo E. et al. Non-Linear Formation of EtG and FAEEs after Controlled Administration of Low to Moderate Doses of Ethanol. Alcohol Alcohol. 2017 Sep 1;52(5):587-594 is available online at: http://dx.doi.org/10.1093/alcalc/agx033
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.subject.keyword Ethanol
dc.subject.keyword Esters
dc.subject.other Marcadors bioquímics
dc.subject.other Alcohol -- Efectes fisiològics
dc.title Non-linear formation of EtG and FAEEs after controlled administration of low to moderate doses of ethanolca
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/acceptedVersion

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