Chromatin remodeling in Drosophila preblastodermic embryo extract

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  • dc.contributor.author Šatović, Eva
  • dc.contributor.author Font Mateu, Jofre, 1977-
  • dc.contributor.author Carbonell, Albert
  • dc.contributor.author Beato, Miguel
  • dc.contributor.author Azorín Marín, Fernando
  • dc.date.accessioned 2019-12-09T09:23:11Z
  • dc.date.available 2019-12-09T09:23:11Z
  • dc.date.issued 2018
  • dc.description.abstract Chromatin is known to undergo extensive remodeling during nuclear reprogramming. However, the factors and mechanisms involved in this remodeling are still poorly understood and current experimental approaches to study it are not best suited for molecular and genetic analyses. Here we report on the use of Drosophila preblastodermic embryo extracts (DREX) in chromatin remodeling experiments. Our results show that incubation of somatic nuclei in DREX induces changes in chromatin organization similar to those associated with nuclear reprogramming, such as rapid binding of the germline specific linker histone dBigH1 variant to somatic chromatin, heterochromatin reorganization, changes in the epigenetic state of chromatin, and nuclear lamin disassembly. These results raise the possibility of using the powerful tools of Drosophila genetics for the analysis of chromatin changes associated with this essential process.
  • dc.description.sponsorship This work was supported by grants from MINECO (BFU2015-65082-P), the Generalitat de Catalunya (SGR2014-204), and the European Community FEDER program. This work was carried out within the framework of the “Centre de Referència en Biotecnologia” of the Generalitat de Catalunya. EŠ acknowledges receipt of a NEWFELPRO Fellowship of the Croatian Government and Ministry of Science and Education within EU FP7 Programme framework.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Šatović E, Font-Mateu J, Carbonell A, Beato M, Azorín F. Chromatin remodeling in Drosophila preblastodermic embryo extract. Sci Rep. 2018;8(1):10927. DOI: 10.1038/s41598-018-29129-8
  • dc.identifier.doi http://dx.doi.org/10.1038/s41598-018-29129-8
  • dc.identifier.issn 2045-2322
  • dc.identifier.uri http://hdl.handle.net/10230/43120
  • dc.language.iso eng
  • dc.publisher Nature Research
  • dc.relation.ispartof Scientific Reports. 2018;8(1):10927
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-65082-P
  • dc.rights © The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword Chromatin
  • dc.subject.keyword Epigenetics
  • dc.title Chromatin remodeling in Drosophila preblastodermic embryo extract
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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