Peptide selection for targeted protein quantitation
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- dc.contributor.author Chiva, Cristinaca
- dc.contributor.author Sabidó Aguadé, Eduard, 1981-ca
- dc.date.accessioned 2018-06-14T07:43:23Z
- dc.date.available 2018-06-14T07:43:23Z
- dc.date.issued 2017
- dc.description.abstract Targeted proteomics methods in their different flavors rely on the use of a few peptides as proxies for protein quantitation, which need to be specified either prior to or after data acquisition. However, in contrast with discovery methods that use all identified peptides for a given protein to estimate its abundance, targeted proteomics methods are limited in the number of peptides that are used for protein quantitation. Because only a few peptides per protein are acquired or extracted in targeted experiments, the selection of peptides that are used for targeted protein quantitation becomes crucial. Several rules have been proposed to guide peptide selection for targeted proteomics studies, which have generally been based on the amino acidic composition of the peptide sequences. However, the compliance of these rules does not imply that not-conformed peptides are not reproducibly generated nor do they guarantee that the selected peptides correctly represent the behavior of the protein abundance under different conditions.
- dc.format.mimetype application/pdf
- dc.identifier.citation Chiva C, Sabidó E. Peptide Selection for Targeted Protein Quantitation. J Proteome Res. 2017 Mar 3;16(3):1376-80. DOI: 10.1021/acs.jproteome.6b00115. Epub 2017 Jan 30
- dc.identifier.doi http://dx.doi.org/10.1021/acs.jproteome.6b00115
- dc.identifier.issn 1535-3893
- dc.identifier.uri http://hdl.handle.net/10230/34897
- dc.language.iso eng
- dc.publisher American Chemical Society (ACS)ca
- dc.relation.ispartof Journal of Proteome Research. 2017 Mar 3;16(3):1376-80
- dc.rights This document is the Accepted Manuscript version of a Published Work that appeared in final form in Proteome Res., copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.jproteome.6b00115
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.keyword Data independent acquisition
- dc.subject.keyword Selected reaction monitoring
- dc.subject.keyword Targeted proteomics
- dc.subject.keyword Quantitation
- dc.title Peptide selection for targeted protein quantitationca
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/acceptedVersion