Mutational profile and pathological features of a case of interleukin-10 and RGS1-positive spindle cCell variant diffuse large B-Cell lymphoma
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- dc.contributor.author Carreras, Joaquim
- dc.contributor.author Kikuti, Yara Yukie
- dc.contributor.author Miyaoka, Masashi
- dc.contributor.author Hiraiwa, Shinichiro
- dc.contributor.author Tomita, Sakura
- dc.contributor.author Ikoma, Haruka
- dc.contributor.author Kondo, Yusuke
- dc.contributor.author Ito, Atsushi
- dc.contributor.author Nagase, Shunsuke
- dc.contributor.author Miura, Hisanobu
- dc.contributor.author Roncador, Giovanna
- dc.contributor.author Colomo Saperas, Luis Alberto
- dc.contributor.author Hamoudi, Rifat
- dc.contributor.author Campo, Elias
- dc.contributor.author Nakamura, Naoya
- dc.date.accessioned 2024-05-23T13:49:24Z
- dc.date.available 2024-05-23T13:49:24Z
- dc.date.issued 2023
- dc.description.abstract Diffuse large B-cell lymphoma with spindle cell morphology is a rare variant. We present the case of a 74-year-old male who initially presented with a right supraclavicular (lymph) node enlargement. Histological analysis showed a proliferation of spindle-shaped cells with narrow cytoplasms. An immunohistochemical panel was used to exclude other tumors, such as melanoma, carcinoma, and sarcoma. The lymphoma was characterized by a cell-of-origin subtype of germinal center B-cell-like (GCB) based on Hans' classifier (CD10-negative, BCL6-positive, and MUM1-negative); EBER negativity, and the absence of BCL2, BCL6, and MYC rearrangements. Mutational profiling using a custom panel of 168 genes associated with aggressive B-cell lymphomas confirmed mutations in ACTB, ARID1B, DUSP2, DTX1, HLA-B, PTEN, and TNFRSF14. Based on the LymphGen 1.0 classification tool, this case had an ST2 subtype prediction. The immune microenvironment was characterized by moderate infiltration of M2-like tumor-associated macrophages (TMAs) with positivity of CD163, CSF1R, CD85A (LILRB3), and PD-L1; moderate PD-1 positive T cells, and low FOXP3 regulatory T lymphocytes (Tregs). Immunohistochemical expression of PTX3 and TNFRSF14 was absent. Interestingly, the lymphoma cells were positive for HLA-DP-DR, IL-10, and RGS1, which are markers associated with poor prognosis in DLBCL. The patient was treated with R-CHOP therapy, and achieved a metabolically complete response.
- dc.format.mimetype application/pdf
- dc.identifier.citation Carreras J, Kikuti YY, Miyaoka M, Hiraiwa S, Tomita S, Ikoma H, et al. Mutational profile and pathological features of a case of interleukin-10 and RGS1-positive spindle cCell variant diffuse large B-Cell lymphoma. Hematol Rep. 2023 Mar 12;15(1):188-200. DOI: 10.3390/hematolrep15010020
- dc.identifier.doi http://dx.doi.org/10.3390/hematolrep15010020
- dc.identifier.issn 2038-8322
- dc.identifier.uri http://hdl.handle.net/10230/60237
- dc.language.iso eng
- dc.publisher MDPI
- dc.relation.ispartof Hematol Rep. 2023 Mar 12;15(1):188-200
- dc.rights © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword LymphGen 1.0
- dc.subject.keyword Diffuse large B-cell lymphoma
- dc.subject.keyword Interleukin 10
- dc.subject.keyword Mutational profiling
- dc.subject.keyword Next-generation sequencing
- dc.subject.keyword Regulator of G protein signaling 1
- dc.subject.keyword Spindle cell
- dc.title Mutational profile and pathological features of a case of interleukin-10 and RGS1-positive spindle cCell variant diffuse large B-Cell lymphoma
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion