Time-resolved gene expression profiling during reprogramming of C/EBPα-pulsed B cells into iPS cells

Di Stefano, Bruno, 1984-; Collombet, Samuel; Graf, T. (Thomas)

doi:http://dx.doi.org/10.1038/sdata.2014.8

Time-resolved gene expression profiling during reprogramming of C/EBPα-pulsed B cells into iPS cells

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Di Stefano B, Collombet S, Graf T. Time-resolved gene expression profiling during reprogramming of C/EBPα-pulsed B cells into iPS cells. Sci Data. 2014 May 27;1(1):140008. DOI: 10.1038/sdata.2014.8

Abstract

The reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) is lengthy and inefficient. The development of a reprogramming system that allows rapid and synchronous reprogramming to pluripotency is imperative for understanding the mechanism of iPSC formation and for future therapeutic applications. We have recently reported that a short expression in mouse primary B cells of the transcription factor C/EBPα before the induction of pluripotency factors increases the iPSC reprogramming efficiency >100-fold, involving 95% of the cells within a week. Here we present a dataset containing the time course of gene expression during this process as determined by microarray and RNA-seq techniques.